Human AK3 antibody | NKMAX AAK0604 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human AK3 antibody

catalog :

AAK0604

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SJB3-36

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human AK3 antibody

Catalog number (SKU) :

AAK0604

NCBI :

NP_057366

Description :

Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

Alternative Names :

adenylate kinase, Ak6, FIX, Ak3L1, AkL3L, AkL3L1, adenylate kinase 3, adenylate kinase 6, adenylate kinase 3 like 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305.;; Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101.;; Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262.;; Lee, Y. et al. (1998) J. Biochem. 123, 47-54.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC, FACS

IF Comment And Pic :

ICC/IF analysis of AK3 in U87MG cells. The cell was stained with AAK0604 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : Recombinant Human AK1;; Lane 2. : Recombinant Human AK2;; Lane 3. : Recombinant Human AK3 The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HepG2 cell lysate;; Lane 2.: Lncap cell lysate;; {2004010017118777.jpg}

Reacts With :

Human

Clone Number :

SJB3-36

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

IHC Comment And Pic :

Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UIJ7

Internal Search Terms :

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more info or order :

NKMAX product webpage