antibody

Human Adiponectin/Acrp30 antibody

AAD0614

50 ul

170 USD

monoclonal

mouse

nonconjugated

5H7

western blot, ELISA, immunocytochemistry

Human Adiponectin/Acrp30 antibody

AAD0614

NP_004788

Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

ADIPOQ, ACDC, ACRP30, APM1, GBP28, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-289.;; Berg AH. et al., (2001) Nat Med. 7:947-953.;; Berg AH. et al., (2002) Trends Endocrinol Metab. 13:84-89.;; Yamauchi T. et al., (2002) Nat Med. 8:1288-1295.

Mouse

IgG1 kappa

Recombinant human adiponectin (15-244aa) purified from E. coli

ELISA, WB, ICC/IF

ICC/IF analysis of Adiponectin in Hep3B cells. The cell was stained with AAD0614 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1. : 293T cell lysate;; Lane 2. : Adiponectin collagen domain(15-107aa) transfected 293T cell lysate;; Lane 3. : Adiponectin Full domain(15-244aa) transfected 293T cell lysate;; Lane 4. : Adiponectin globular domain(108-244aa) transfected 293T cell lysate

Human

5H7

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Hormones & Cytokines

Unconjugated

Monoclonal

By protein-G affinity chromatography

Monoclonal Antibody

Q15848

Adiponectin, Acrp30,AAD0614-10ug,AAD0614-20ug,AAD0614-50ug,AAD0614-100ug,AAD0614-250ug,AAD0614-500ug,AAD0614-1mg,AAD0614-10,AAD0614-20,AAD0614-50,AAD0614-100,AAD0614-250,AAD0614-500,AAD0614-1

Almer G, et al. Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. (Int J Nanomedicine. 2011){https://pubmed.ncbi.nlm.nih.gov/21753879/};;Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. (Biologics. 2011){https://pubmed.ncbi.nlm.nih.gov/22022204/}