protein

Recombinant dengue virus 2 Envelope protein

ATGP2755

20 ug

105 USD

Recombinant dengue virus 2 Envelope protein

298-400aa

ATGP2755

AAA42954

Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. This protein is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes. Recombinant Dengue virus DENV protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dengue virus 2 envelope protein

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Blok J., et al. (1989) Arch. Virol. 105:39-53

> 95% by SDS-PAGE

SDS-PAGE

Dengue virus 2

Virus

13.8 kDa (126aa)

SYSMCTG KFKVVKEIAE TQHGTIVIRV QYEGDGSPCK IPFEIMDLEK RHVLGRLITV NPIVTEKDSP VNIEAEPPFG DSYIIIGVEP GQLKLNWFKK GSSIGQ

His-Tag

E.coli

Recombinant Protein

Q88631

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