FAK (Phospho Tyr397) Polyclonal Antibody | MyBioSource MBS9706532 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FAK (Phospho Tyr397) Polyclonal Antibody

catalog :

MBS9706532

quantity :

0.03 mL

price :

130 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Fig.1. Western Blot analysis of 293 cells using Phospho-FAK (Y397) Polyclonal Antibody.

image 2 :

Fig.2. Western Blot analysis of 293 cells using Phospho-FAK (Y397) Polyclonal Antibody.

product information

catalog number :

MBS9706532

products type :

Antibody

products full name :

FAK (Phospho Tyr397) Polyclonal Antibody

products short name :

[FAK]

products name syn :

[Rabbit Anti-FAK (phospho Tyr397) Polyclonal Antibody; PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK]

other names :

[focal adhesion kinase 1 isoform c; Focal adhesion kinase 1; focal adhesion kinase 1; protein tyrosine kinase 2; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK]

products gene name :

[FAK]

other gene names :

[PTK2; PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; FAK; FAK1; FADK 1; FRNK; PPP1R71]

uniprot entry name :

FAK1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1065

form :

Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

concentration :

1 mg/ml

storage stability :

Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

products description :

PTK2 (protein tyrosine kinase 2) encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of PTK2 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined.

ncbi gi num :

313851044

ncbi acc num :

NP_001186578.1

ncbi gb acc num :

NP_001186578.1

uniprot acc num :

Q05397

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Alpha 6 Beta 4 Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198806); Angiogenesis Pathway (198772); Apoptosis Pathway (1270262); Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway (1458189); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287)

ncbi summary :

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

uniprot summary :

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed. Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fak family. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; cytoskeleton; lamellipodium; cytoplasm; apical plasma membrane; stress fiber; plasma membrane; microtubule organizing center; cell cortex; cytosol; nucleus. Molecular Function: JUN kinase binding; protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity. Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; establishment of nucleus localization; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; cell motility involved in cell locomotion; neuron migration; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; cell structure disassembly during apoptosis; placenta development; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; regulation of osteoblast differentiation; central nervous system neuron axonogenesis; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; endothelial cell migration; innate immune response; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration; regulation of cytoskeleton organization and biogenesis; negative regulation of apoptosis

size1 :

0.03 mL

price1 :

130 USD

size2 :

0.1 mL

price2 :

220

size3 :

0.2 mL

price3 :

325

more info or order :

MyBioSource product webpage