Recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | MyBioSource MBS965770 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)

catalog :

MBS965770

quantity :

0.5 mg (E-Coli)

price :

1095 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS965770

products type :

Recombinant Protein

products full name :

Recombinant Mouse Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)

products short name :

Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)

products name syn :

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; EC=1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-

other names :

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

products gene name :

Gapdh

products gene name syn :

Gapdh; Gapd

other gene names :

Gapdh; Gapdh; Gapd; Gapd; GAPDH

uniprot entry name :

G3P_MOUSE

host :

E Coli or Yeast or Baculovirus or Mammalian Cell

sequence positions :

2-333

sequence length :

332

sequence :

VKVGVNGFG RIGRLVTRAA ICSGKVEIVA INDPFIDLNY MVYMFQYDST HGKFNGTVKA ENGKLVINGK PITIFQERDP TNIKWGEAGA EYVVESTGVF TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIVSNASC TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP AKYDDIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSNSHS STFDAGAGIA LNDNFVKLIS WYDNEYGYSN RVVDLMAYMA SKE

purity :

>90%

form :

Liquid containing glycerol; lyophilization may be available upon request.

storage stability :

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

other info1 :

Species: Mus musculus (Mouse)

ncbi gi num :

576080555

ncbi acc num :

NP_001276655.1

ncbi gb acc num :

NM_001289726.1

uniprot acc num :

P16858

ncbi mol weight :

35,810 Da

ncbi pathways :

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Biosynthesis Of Amino Acids Pathway (790952); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815838); Carbon Metabolism Pathway (817567); Disease Pathway (970606); Gluconeogenesis Pathway (970554); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (421742); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014]

uniprot summary :

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma. Protein type: EC 1.2.1.12; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; nucleus; cytosol; vesicle. Molecular Function: identical protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity. Biological Process: neuron apoptosis; glycolysis; protein stabilization; apoptosis; multicellular organismal development; negative regulation of translation; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis

size1 :

0.5 mg (E-Coli)

price1 :

1095 USD

size2 :

0.05 mg (Baculovirus)

price2 :

1095

size3 :

0.5 mg (Yeast)

price3 :

1325

size4 :

0.05 mg (Mammalian-Cell)

price4 :

1325

size5 :

1 mg (E-Coli)

price5 :

1725

more info or order :

MyBioSource product webpage