Recombinant Bovine Beta-1,4-galactosyltransferase 1 | MyBioSource MBS963398 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Bovine Beta-1,4-galactosyltransferase 1

catalog :

MBS963398

quantity :

0.05 mg (E-Coli)

price :

190 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS963398

products type :

Recombinant Protein

products full name :

Recombinant Bovine Beta-1,4-galactosyltransferase 1

products short name :

Beta-1,4-galactosyltransferase 1

products name syn :

UDP-Gal: beta-GlcNAc beta-1,4-galactosyltransferase 1

other names :

beta-1,4-galactosyltransferase 1; Beta-1,4-galactosyltransferase 1; beta-1,4-galactosyltransferase 1; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

products gene name :

B4GALT1

other gene names :

B4GALT1; B4GALT1; GGTB2; GALT; GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1

uniprot entry name :

B4GT1_BOVIN

host :

E Coli or Yeast or Baculovirus or Mammalian Cell

sequence positions :

24-Jan

sequence length :

402

sequence :

MKFREPLLGGSAAMPGASLQRACR

purity :

Greater than 90% as determined by SDS-PAGE.

form :

Liquid containing glycerol; lyophilization may be available upon request.

storage stability :

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

products description :

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

products references :

Cloning of cDNA encoding the membrane-bound form of bovine beta 1,4-galactosyltransferase.D'Agostaro G., Bendiak B., Tropak M.Eur. J. Biochem. 183:211-217(1989) NIH - Mammalian Gene Collection (MGC) project Bovine galactosyltransferase identification of a clone by direct immunological screening of a cDNA expression library.Shaper N.L., Shaper J.H., Meuth J.L., Fox J.L., Chang H., Kirsch I.R., Hollis G.F.Proc. Natl. Acad. Sci. U.S.A. 83:1573-1577(1986) Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4) galactosyltransferase.Narimatsu H., Sinha S., Brew K., Okayama H., Qasba P.K.Proc. Natl. Acad. Sci. U.S.A. 83:4720-4724(1986) Qasba P.K., Narimatsu H., Masibay A.S.Expression of bovine beta-1,4-galactosyltransferase cDNA in COS-7 cells.Masibay A.S., Qasba P.K.Proc. Natl. Acad. Sci. U.S.A. 86:5733-5737(1989) Bovine beta 1-->4-galactosyltransferase two sets of mRNA transcripts encode two forms of the protein with different amino-terminal domains. In vitro translation experiments demonstrate that both the short and the long forms of the enzyme are type II membrane-bound glycoproteins.Russo R.N., Shaper N.L., Shaper J.H.J. Biol. Chem. 265:3324-3331(1990) Identification of a region of UDP-galactose:N-acetylglucosamine beta 4-galactosyltransferase involved in UDP-galactose binding by differential labeling.Yadav S.P., Brew K.J. Biol. Chem. 265:14163-14169(1990) Structure and function in galactosyltransferase. Sequence locations of alpha-lactalbumin binding site, thiol groups, and disulfide bond.Yadav S.P., Brew K.J. Biol. Chem. 266:698-703(1991) Crystal structures of the bovine beta4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose.Gastinel L.N., Cambillau C., Bourne Y.EMBO J. 18:3546-3557(1999) Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1,4-galactosyltransferase-I.Ramakrishnan B., Qasba P.K.J. Mol. Biol. 310:205-218(2001) Crystal structure of beta1,4-galactosyltransferase complex with UDP-Gal reveals an oligosaccharide acceptor binding site.Ramakrishnan B., Balaji P.V., Qasba P.K.J. Mol. Biol. 318:491-502(2002) The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I.Ramasamy V., Ramakrishnan B., Boeggeman E., Qasba P.K.J. Mol. Biol. 331:1065-1076(2003)

ncbi gi num :

29135321

ncbi acc num :

NP_803478.1

ncbi gb acc num :

NM_177512.2

uniprot acc num :

P08037

ncbi mol weight :

29.95kD

ncbi pathways :

Asparagine N-linked Glycosylation Pathway (1331710); Galactose Metabolism Pathway (84177); Galactose Metabolism Pathway (292); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (84226); Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway (357); Glycosaminoglycan Metabolism Pathway (1331819); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (84238); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370); Glycosphingolipid Biosynthesis, Neolacto-series, LacCer = NLc4Cer Pathway (391006); Glycosphingolipid Biosynthesis, Neolacto-series, LacCer = NLc4Cer Pathway (468264)

size1 :

0.05 mg (E-Coli)

price1 :

190 USD

size2 :

0.2 mg (E-Coli)

price2 :

460

size3 :

0.5 mg (E-Coli)

price3 :

750

size4 :

1 mg (E-Coli)

price4 :

1180

size5 :

1 mg (Yeast)

price5 :

1430

more info or order :

MyBioSource product webpage