CHRNG Antibody | MyBioSource MBS9609461 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CHRNG Antibody

catalog :

MBS9609461

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from Mouse muscle, using CHRNG Ab. Lane 1 was treated with the blocking peptide.

image 2 :

Western blot analysis of extracts from HepG2, using CHRNG Ab.

image 3 :

Western blot analysis of CHRNG using HT29 whole cell lysates

product information

catalog number :

MBS9609461

products type :

Antibody

products full name :

CHRNG Antibody

products short name :

[CHRNG]

products name syn :

[Acetylcholine receptor muscle gamma subunit; Acetylcholine receptor protein gamma chain precursor; Acetylcholine receptor subunit gamma; ACHG; ACHG_HUMAN; Achr 3; AChR; Achr3; ACHRG; ACRG; Cholinergic receptor nicotinic gamma; Cholinergic receptor nicotinic gamma polypeptide; CHRNG; MGC133376]

other names :

[acetylcholine receptor subunit gamma; Acetylcholine receptor subunit gamma; acetylcholine receptor subunit gamma; cholinergic receptor nicotinic gamma subunit]

products gene name :

[CHRNG]

other gene names :

[CHRNG; CHRNG; ACHRG; ACHRG]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

517

specificity :

CHRNG antibody detects endogenous levels of total CHRNG

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:1000-3000 IHC: 1:200

image1 heading :

Western blot

image2 heading :

Western blot

image3 heading :

Western blot

image4 heading :

Immunohistochemistry

image4 description :

MBS9609461 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

other info1 :

Immunogen: A synthesized peptide derived from human CHRNG. Subcellular Location: Cell Junction > Synapse > Postsynaptic Cell Membrane. Cell Membrane. Predicted Cross Reactivity : Pig, Bovine, Horse, Sheep, Rabbit, Dog . Similarity: Pig (90%), Bovine (90%), Horse (100%), Sheep (90%), Rabbit (90%), Dog (90%)

other info2 :

Conjugation: Unconjugated. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

products description :

Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Subunit Structure: Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains. Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Gamma/CHRNG sub-subfamily.

ncbi gi num :

61743914

ncbi acc num :

NP_005190.4

ncbi gb acc num :

NM_005199.5

uniprot acc num :

P07510

ncbi mol weight :

Observed: 58 kDa. Predicted: 58 kDa

ncbi pathways :

Acetylcholine Binding And Downstream Events Pathway (106535); Activation Of Nicotinic Acetylcholine Receptors Pathway (161027); Highly Sodium Permeable Acetylcholine Nicotinic Receptors Pathway (161030); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Postsynaptic Nicotinic Acetylcholine Receptors Pathway (161031); Presynaptic Nicotinic Acetylcholine Receptors Pathway (161028); Transmission Across Chemical Synapses Pathway (106516)

ncbi summary :

The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009]

uniprot summary :

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage