MaxiKbeta Antibody | MyBioSource MBS9608888 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MaxiKbeta Antibody

catalog :

MBS9608888

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9608888 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

Western blot analysis of MaxiKbeta using HT29 whole cell lysates

image 3 :

Western blot analysis of extracts from COS-7, using MaxiKBeta Ab. The lane on the left was treated with blocking peptide.

product information

catalog number :

MBS9608888

products type :

Antibody

products full name :

MaxiKbeta Antibody

products short name :

[MaxiKbeta]

products name syn :

[BK channel subunit beta 4; BK channel subunit beta-4; BKbeta4; Calcium activated potassium channel beta 4 subunit; Calcium activated potassium channel subfamily M subunit beta 4; Calcium activated potassium channel subunit beta 4; Calcium-activated potassium channel; Calcium-activated potassium channel subunit beta-4; Charybdotoxin receptor subunit beta 4; Charybdotoxin receptor subunit beta-4; Hbeta4; K(VCA)beta 4; K(VCA)beta-4; KCMB4_HUMAN; KCNMB 4; Kcnmb4; Large conductance calcium dependent potassium ion channel beta 4 subunit; Maxi K channel subunit beta 4; Maxi K channel subunit beta-4; Potassium large conductance calcium activated channel subfamily M beta member 4; Slo beta 4; Slo-beta-4; subfamily M subunit beta-4]

other names :

[calcium-activated potassium channel subunit beta-4; Calcium-activated potassium channel subunit beta-4; calcium-activated potassium channel subunit beta-4; potassium calcium-activated channel subfamily M regulatory beta subunit 4; BK channel subunit beta-4; BKbeta4; Hbeta4; Calcium-activated potassium channel, subfamily M subunit beta-4; Charybdotoxin receptor subunit beta-4; K(VCA)beta-4; Maxi K channel subunit beta-4; Slo-beta-4]

products gene name :

[KCNMB4]

other gene names :

[KCNMB4; KCNMB4; BKbeta4; Hbeta4]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat, Monkey

sequence length :

210

specificity :

MaxiKbeta antibody detects endogenous levels of total MaxiKbeta

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:1000-3000. IHC: 1:200

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human MaxiK beta, corresponing to a region within the internal amino acids

other info2 :

Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

products description :

Function: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations. Subunit Structure: Interacts with KCNMA1 tetramer (PubMed:10692449, PubMed:10804197). There are probably 4 molecules of KCMNB4 per KCNMA1 tetramer (PubMed:10692449, PubMed:10804197). Interacts with FMR1 (via N-terminus) (PubMed:25561520). Post-translational Modifications: Phosphorylated. Phosphorylation modulates its effect on KCNMA1 activation kinetics. N-glycosylated. A highly glycosylated form is promoted by KCNMA1. Glycosylation, which is not required for the interaction with KCNMA1 and subcellular location, increases protection against charybdotoxin. Similarity: Resistance to charybdotoxin (CTX) toxin is mediated by the extracellular domain. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB4 subfamily.

ncbi gi num :

26051275

ncbi acc num :

NP_055320.4

ncbi gb acc num :

NM_014505.5

uniprot acc num :

Q86W47

ncbi mol weight :

23 kDa

ncbi pathways :

Ca2+ Activated K+ Channels Pathway (1268822); Hemostasis Pathway (1269340); Insulin Secretion Pathway (777534); Neuronal System Pathway (1268763); Nitric Oxide Stimulates Guanylate Cyclase Pathway (1269343); Platelet Homeostasis Pathway (1269341); Potassium Channels Pathway (1268821); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866); CGMP Effects Pathway (1269344)

ncbi summary :

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit. [provided by RefSeq, Jul 2008]

uniprot summary :

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage