WRN Antibody | MyBioSource MBS9608745 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

WRN Antibody

catalog :

MBS9608745

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9608745 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

Western blot analysis of WRN using RAW264.7 whole cell lysates

image 3 :

MBS9608745 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

product information

catalog number :

MBS9608745

products type :

Antibody

products full name :

WRN Antibody

products short name :

[WRN]

products name syn :

[DKFZp686C2056; DNA helicase; DNA helicase, RecQ like type 3; Exonuclease WRN; HGNC 12791; OTTHUMP00000225301; RecQ protein-like 2; RecQ-like type 3; RecQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome helicase; Werner syndrome protein; Werner syndrome, RecQ helicase like; WRN; WRN_HUMAN]

other names :

[Werner syndrome ATP-dependent helicase; Werner syndrome ATP-dependent helicase; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; DNA helicase, RecQ-like type 3; RecQ3; Exonuclease WRN (EC:3.1.-.-); RecQ protein-like 2]

products gene name :

[WRN]

other gene names :

[WRN; WRN; RECQ3; RECQL2; RECQL3; RECQ3; RECQL2; RecQ3]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1432

specificity :

WRN antibody detects endogenous levels of total WRN

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:1000-3000. IHC: 1:200. IF/ICC: 1:100-1:500

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: A synthesized peptide derived from human WRN

other info2 :

Similarity: Belongs to the helicase family. RecQ subfamily.

products description :

Function: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'-> 5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. Subunit Structure: Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1 (By similarity). Interacts with EXO1, PCNA and SUPV3L1. Interacts with PML (isoform PML-4). Post-translational Modifications: Phosphorylated by PRKDC. Similarity: Belongs to the helicase family. RecQ subfamily.

ncbi gi num :

110735439

ncbi acc num :

NP_000544.2

ncbi gb acc num :

NM_000553.5

uniprot acc num :

Q14191

ncbi mol weight :

162 kDa

ncbi pathways :

Integrated Pancreatic Cancer Pathway (711360); Regulation Of Telomerase Pathway (137987)

ncbi summary :

This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers. [provided by RefSeq, Aug 2017]

uniprot summary :

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (). Plays a role in double-strand break repair after gamma-irradiation.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage