Phospho-nrf2(Ser40) Antibody | MyBioSource MBS9608128 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-nrf2(Ser40) Antibody

catalog :

MBS9608128

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Phospho-nrf2(S40) using HT-29 whole cell lysates

image 2 :

MBS9608128 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 3 :

MBS9608128 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

product information

catalog number :

MBS9608128

products type :

Antibody

products full name :

Phospho-nrf2(Ser40) Antibody

products short name :

[nrf2]

products name syn :

[erythroid derived 2; HEBP1; like 2; NF E2 related factor 2; NF-E2-related factor 2; NF2L2_HUMAN; NFE2 related factor 2; NFE2-related factor 2; Nfe2l2; Nrf 2; NRF2; Nuclear factor (erythroid derived 2) like 2; Nuclear factor; nuclear factor erythroid 2 like 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid 2-related factor 2; Nuclear factor erythroid derived 2 like 2]

other names :

[nuclear factor erythroid 2-related factor 2 isoform 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; nuclear factor, erythroid 2 like 2; HEBP1; Nuclear factor, erythroid derived 2, like 2]

products gene name :

[nrf2]

products gene name syn :

[NFE2L2]

other gene names :

[NFE2L2; NFE2L2; NRF2; HEBP1; IMDDHH; NRF2; NF-E2-related factor 2; NFE2-related factor 2]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

589

specificity :

Phospho-nrf2(S40) antibody detects endogenous levels of nrf2 only when phosphorylated at S40

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

form :

Rabbit igG in phosphate buffered saline, pH 7.4, 150 mM NaCI, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:1000-3000. IHC: 1:200. IF: 1:100-1:500. For Western Blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween R 20 at 4°C with gentle shaking, overnigth.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: A synthesized peptide derived from human Phospho-nrf2(S40) . Similarity: Acidic activation domain in the N-terminus and DNA binding domaing in the C-terminus. Belongs to the bZIP family. CNC subfamily. Subcellular Location: Cytoplasm > Cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Predicted Cross Reactivity : Pig, Bovine, Horse, Rabbit, Dog, Chicken . Similarity: Pig (100%), Bovine (91%), Horse (89%), Rabbit (82%), Dog (100%), Chicken (91%)

products description :

Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit Structure: Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Interacts with EEF1D at heat shock promoter elements (HSE). Interacts (via the bZIP domain) with MAFK; required for binding to antioxidant response (ARE) elements on DNA. Interacts with CHD6; involved in activation of the transcription. Interacts with ESRRB; represses NFE2L2 transcriptional activity (By similarity). Post-translational Modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane. Similarity: Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. Belongs to the bZIP family. CNC subfamily.

ncbi gi num :

224028257

ncbi acc num :

NP_001138884.1

ncbi gb acc num :

NM_001145412.3

uniprot acc num :

Q16236

ncbi mol weight :

Observed: 90 kDa. Predicted: 68 kDa

ncbi pathways :

AhR Pathway (755436); Keap1-Nrf2 Pathway (198777); Oxidative Stress Pathway (198916); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

uniprot summary :

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress and the regulation of cellular redox conditions. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage