Phospho-ATR(Ser428) Antibody | MyBioSource MBS9608121 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-ATR(Ser428) Antibody

catalog :

MBS9608121

quantity :

0.1 mL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

citations: 1

Reference
Muralimanoharan S, Shamby R, Stansbury N, Schenken R, de la Peña Avalos B, Javanmardi S, et al. Aberrant R-loop-induced replication stress in MED12-mutant uterine fibroids. Sci Rep. 2022;12:6169 pubmed publisher

image

image 1 :

Western blot analysis of phospho-ATR(S428) using COLO205 whole cell lysates

image 2 :

MBS9608121 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

product information

catalog number :

MBS9608121

products type :

Antibody

products full name :

Phospho-ATR(Ser428) Antibody

products short name :

[ATR]

products name syn :

[Ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR; ATR_HUMAN; FCTCS; FRAP Related Protein 1; FRAP-related protein 1; FRP1; MEC1; MEC1 mitosis entry checkpoint 1 homolog; Protein kinase ATR; RAC3; Rad3 related protein; SCKL; SCKL1; Serine/threonine protein kinase ATR; Serine/threonine-protein kinase ATR]

other names :

[serine/threonine-protein kinase ATR isoform 1; Serine/threonine-protein kinase ATR; serine/threonine-protein kinase ATR; ATR serine/threonine kinase; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1]

products gene name :

[ATR]

other gene names :

[ATR; ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1; FRP1]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

2644

specificity :

phospho-ATR(S428) antibody detects endogenous levels of ATR only when phosphorylated at S428

purity :

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:1000-3000. IHC: 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: A synthesized peptide derived from human phospho-ATR(S428)

products description :

Function: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Similarity: Belongs to the PI3/PI4-kinase family. ATM subfamily.

ncbi gi num :

157266317

ncbi acc num :

NP_001175.2

ncbi gb acc num :

NM_001184.3

uniprot acc num :

Q13535

ncbi mol weight :

300 kDa

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chromosome Maintenance Pathway (161048); Circadian Rhythm Pathway (137978); DNA Repair Pathway (105837)

ncbi summary :

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]

uniprot summary :

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

size1 :

0.1 mL

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage