POLR2J Antibody | MyBioSource MBS9607309 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

POLR2J Antibody

catalog :

MBS9607309

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9607309 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

image 2 :

Western blot analysis of K562 whole cell lysates, using POLR2J Antibody. The lane on the left is treated with the antigen-specific peptide.

image 3 :

MBS9607309 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

product information

catalog number :

MBS9607309

products type :

Antibody

products full name :

POLR2J Antibody

products short name :

[POLR2J]

products name syn :

[DNA directed RNA polymerase II subunit RPB11 a; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II subunit J 1; DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11-a; hRPB 14; hRPB14; POLR 2J1; POLR2J; POLR2J1; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; RNA polymerase II 13.3 kDa subunit; RNA polymerase II subunit B11 a; RNA polymerase II subunit B11-a; RPB 11; RPB 11A; RPB 11m; RPB11; RPB11_HUMAN; RPB11a; RPB11m; Rpo2-4]

other names :

[DNA-directed RNA polymerase II subunit RPB11-a; DNA-directed RNA polymerase II subunit RPB11-a; DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit J; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit]

products gene name :

[POLR2J]

other gene names :

[POLR2J; POLR2J; RPB11; RPB11A; RPB11m; hRPB14; POLR2J1; POLR2J1; RNA polymerase II subunit B11-a; RPB11a]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

117

specificity :

POLR2J antibody detects endogenous levels of total POLR2J

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IF/ICC: 1:100-1:500

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: A synthesized peptide derived from human POLR2J corresponding to a region within N-terminal amino acids.

products description :

Description: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Similarity: Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

ncbi gi num :

5453932

ncbi acc num :

NP_006225.1

ncbi gb acc num :

NM_006234.4

uniprot acc num :

P52435

ncbi mol weight :

13 kDa

ncbi pathways :

Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway (106005); DNA Repair Pathway (105837); Disease Pathway (530764); Dual Incision Reaction In TC-NER Pathway (105892); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Eukaryotic Transcription Initiation Pathway (198917); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway (161052); Formation Of RNA Pol II Elongation Complex Pathway (105950)

ncbi summary :

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]

uniprot summary :

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft ().

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage