CXADR Antibody | MyBioSource MBS9607294 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CXADR Antibody

catalog :

MBS9607294

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9607294 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

image 2 :

Western blot analysis of HeLa whole cell lysate, using CXADR Antibody. The lane on the left is treated with the antigen-specific peptide.

image 3 :

MBS9607294 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

product information

catalog number :

MBS9607294

products type :

Antibody

products full name :

CXADR Antibody

products short name :

[CXADR]

products name syn :

[46 kD coxsackievirus and adenovirus receptor CAR protein; 46 kD coxsackievirus and adenovirus receptor protein; CAR; CAR4/6; Coxsackie virus and adenovirus receptor; Coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3 binding protein; CVB3-binding protein; Cxadr; CXAR_HUMAN; hCAR; HCVADR]

other names :

[coxsackievirus and adenovirus receptor isoform 2; Coxsackievirus and adenovirus receptor; coxsackievirus and adenovirus receptor; CXADR, Ig-like cell adhesion molecule; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR]

products gene name :

[CXADR]

other gene names :

[CXADR; CXADR; CAR; HCAR; CAR4/6; CAR; CAR; hCAR]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

252

specificity :

CXADR antibody detects endogenous levels of total CXADR

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:2000. IF/ICC: 1:100-1:500. IHC: 1:50-1:200

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

MBS9607294 staining HEPG2 cells by ICC/IF. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.

other info1 :

Immunogen: A synthesized peptide derived from human CXADR. Subcellular Location: Secreted and Cell Membrane. Cell Junction > Tight Junction. Cell Junction > Adherens Junction. Basolateral Cell Membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. Tissue Specificity: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.

other info2 :

Conjugation: Unconjugated

products description :

Description: The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. Function: Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. Subunit Structure: Monomer. May form homodimer. Interacts with LNX, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Interacts with MPDZ; recruits MPDZ to intercellular contact sites. Interacts with JAML (homodimeric form). Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Post-translational Modifications: N-glycosylated. Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. Similarity: The Ig-like C2-type 1 domain mediates homodimerization and interaction with JAML.The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.

ncbi gi num :

333470703

ncbi acc num :

NP_001193992.1

ncbi gb acc num :

NM_001207063.1

uniprot acc num :

P78310

ncbi mol weight :

40 kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Viral Myocarditis Pathway (125138)

ncbi summary :

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

uniprot summary :

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage