SERPINF1 Antibody | MyBioSource MBS9607203 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SERPINF1 Antibody

catalog :

MBS9607203

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from various samples, using SERPINF1 Antibody. lane 1: mouse brain; lane 2: Hela;

image 2 :

MBS9607203 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 3 :

MBS9607203 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

product information

catalog number :

MBS9607203

products type :

Antibody

products full name :

SERPINF1 Antibody

products short name :

[SERPINF1]

products name syn :

[Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; OI12; OI6; PEDF; PEDF_HUMAN; PIG 35; PIG35; Pigment epithelium derived factor; Pigment epithelium-derived factor; Proliferation inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Serpin F1; Serpin family F member 1; Serpin peptidase inhibitor clade F member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; SERPINF 1; Serpinf1]

other names :

[pigment epithelium-derived factor isoform 1; Pigment epithelium-derived factor; pigment epithelium-derived factor; serpin family F member 1; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1]

products gene name :

[SERPINF1]

other gene names :

[SERPINF1; SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35; PEDF; PEDF]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

418

specificity :

SERPINF1 antibody detects endogenous levels of total SERPINF1

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IF/ICC: 1:100-1:500

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of extracts of human liver, using SERPINF1 antibody. The lane on the left is treated with the antigen-specific peptide.

other info1 :

Immunogen: A synthesized peptide derived from human SERPINF1, corresponding to a region within C-terminal amino acids. Similarity: The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Belongs to the serpin family.

products description :

Pigment epithelium-derived growth factor (PEDF), also known as EPC-1 (early population doubling level cDNA-1), is a glycoprotein found naturally in the normal eye. PEDF has reported neuroprotective and differentiation properties and is secreted in abundance by retinal pigment epithelium cells. It belongs to the serine protease inhibitor (Serpin) superfamily and has been reported to inhibit angiogenesis and proliferation of several cell types. The "pooling" of PEDF within the interphotoreceptor matrix places this molecule in a prime physical location to affect the underlying neural retina. Additionally, PEDF induces neuronal differentiation and promotes survival of neurons of the central nervous system from degeneration caused by serum withdrawal or glutamate cytotoxicity.

ncbi gi num :

1049480189

ncbi acc num :

NP_001316832.1

ncbi gb acc num :

NM_001329903.1

uniprot acc num :

P36955

ncbi mol weight :

46 kDa

ncbi pathways :

Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

ncbi summary :

This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]

uniprot summary :

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage