FZD10 Antibody | MyBioSource MBS9606335 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FZD10 Antibody

catalog :

MBS9606335

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9606335 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

image 2 :

Western blot analysis of extracts from COS-7 cells, using FZD10 antibody.The lane on the left is treated with the antigen-specific peptide.

product information

catalog number :

MBS9606335

products type :

Antibody

products full name :

FZD10 Antibody

products short name :

[FZD10]

products name syn :

[CD 350; CD 350 antigen; CD350; Frizzled (Drosophila) homolog 10; Frizzled-10; FZ 10; Fz-10; FZ10; FZD 10; FZD10; FZD10_HUMAN; FzE 7; FzE7; hFz 10; hFz10]

other names :

[frizzled-10; Frizzled-10; frizzled-10; frizzled class receptor 10; FzE7; CD_antigen: CD350]

products gene name :

[FZD10]

other gene names :

[FZD10; FZD10; Fz10; FzE7; CD350; FZ-10; hFz10; Fz-10; hFz10]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Monkey

sequence length :

581

specificity :

FZD10 antibody detects endogenous levels of total FZD10

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:1000. IF/ICC: 1:100-1:500

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human FZD10, corresponding to a region within the internal amino acids.

products description :

Function: Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Similarity: Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.The FZ domain is involved in binding with Wnt ligands. Belongs to the G-protein coupled receptor Fz/Smo family.

ncbi gi num :

6005762

ncbi acc num :

NP_009128.1

ncbi gb acc num :

NM_007197.3

uniprot acc num :

Q9ULW2

ncbi mol weight :

60 kD

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

ncbi summary :

This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008]

uniprot summary :

Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway (). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage