EIF3F Antibody | MyBioSource MBS9605508 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EIF3F Antibody

catalog :

MBS9605508

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9605508 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

MBS9605508 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

image 3 :

Western blot analysis of extracts from Jurkat cells using EIF3F antibody.

product information

catalog number :

MBS9605508

products type :

Antibody

products full name :

EIF3F Antibody

products short name :

[EIF3F]

products name syn :

[Deubiquitinating enzyme eIF3f; eIF 3 epsilon; eIF-3-epsilon; eIF3 p47; eIF3 p47 subunit; eIF3-epsilon; eIF3-p47; eIF3f; EIF3F_HUMAN; EIF3S5; EIF3S5, formerly; Eukaryotic translation initiation factor 3 subunit 5; Eukaryotic translation initiation factor 3 subunit 5, formerly; Eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit F; translation initiation factor 3 47 kda subunit]

other names :

[eukaryotic translation initiation factor 3 subunit F; Eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3 subunit F; Deubiquitinating enzyme eIF3f (EC:3.4.19.12); Eukaryotic translation initiation factor 3 subunit 5UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF-3-epsilonUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF3 p47UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005]

products gene name :

[EIF3F]

other gene names :

[EIF3F; EIF3F; EIF3S5; eIF3-p47; EIF3S5UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF3f]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

357

specificity :

EIF3F antibody detects endogenous levels of total EIF3F

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:1000. IHC: 1:50-1:200. IF/ICC: 1:100-1:500

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescene (IF)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human EIF3F, corresponding to a region within the internal amino acids.

products description :

Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Similarity: The MPN domain mediates deubiquitinating activity. Belongs to the eIF-3 subunit F family.

ncbi gi num :

4503519

ncbi acc num :

NP_003745.1

ncbi gb acc num :

NM_003754.2

uniprot acc num :

O00303

ncbi mol weight :

38 KD

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Cap-dependent Translation Initiation Pathway (105967); Eukaryotic Translation Initiation Pathway (105966); Formation Of A Pool Of Free 40S Subunits Pathway (105968); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (105969); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973); Gene Expression Pathway (105937); L13a-mediated Translational Silencing Of Ceruloplasmin Expression Pathway (160955); Metabolism Of Proteins Pathway (106230); RNA Transport Pathway (177876)

uniprot summary :

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage