MYLIP Antibody | MyBioSource MBS9605476 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MYLIP Antibody

catalog :

MBS9605476

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9605476 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

Western blot analysis of extracts from HeLa cells using MYLIP antibody.

image 3 :

MBS9605476 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

product information

catalog number :

MBS9605476

products type :

Antibody

products full name :

MYLIP Antibody

products short name :

[MYLIP]

products name syn :

[BM-023; BZF1; E3 ubiquitin protein ligase MYLIP; E3 ubiquitin-protein ligase MYLIP; Idol; Inducible degrader of the LDL-receptor; Lipoprotein receptor; MIR; MYLIP; MYLIP_HUMAN; Myosin regulatory light chain interacting protein; PP5242]

other names :

[E3 ubiquitin-protein ligase MYLIP; E3 ubiquitin-protein ligase MYLIP; E3 ubiquitin-protein ligase MYLIP; myosin regulatory light chain interacting protein; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR; RING-type E3 ubiquitin transferase MYLIPCurated]

products gene name :

[MYLIP]

other gene names :

[MYLIP; MYLIP; MIR; IDOL; BZF1; IDOL; Idol; MIR]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

445

specificity :

MYLIP antibody detects endogenous levels of total MYLIP

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500~1:1000. IHC: 1:50~1:200. IF/ICC: 1:100-1:500

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: A synthesized peptide. Subcellular Location: Cytoplasm

products description :

Function:. E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. Similarity:. The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.The FERM domain binds phospholipids and mediates lipoprotein receptors recognition at the plasma membrane through their cytoplasmic tails.The RING-type zinc finger mediates the interaction with UBE2D E2 enzymes.

ncbi gi num :

38788243

ncbi acc num :

NP_037394.2

ncbi gb acc num :

NM_013262.3

uniprot acc num :

Q8WY64

ncbi mol weight :

50 kD

ncbi summary :

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

uniprot summary :

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage