MLH1 Antibody | MyBioSource MBS9605077 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MLH1 Antibody

catalog :

MBS9605077

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from K562 cells, using MLH1 antibody.The lane on the left is treated with the antigen-specific peptide.

image 2 :

MBS9605077 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

image 3 :

Western blot analysis of extracts from mouse lung, using MLH1 Antibody.

product information

catalog number :

MBS9605077

products type :

Antibody

products full name :

MLH1 Antibody

products short name :

[MLH1]

other names :

[DNA mismatch repair protein Mlh1 isoform 1; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1; mutL homolog 1; MutL protein homolog 1]

products gene name :

[MLH1]

other gene names :

[MLH1; MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2; COCA2]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

756

specificity :

MLH1 antibody detects endogenous levels of total MLH1

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:1000. IF/ICC: 1:100-1:500. IMPORTANT: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1 x TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescene (IF)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human MLH1, corresponding to a region within the internal amino acids.

products description :

Function: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. Similarity: Belongs to the DNA mismatch repair MutL/HexB family.

ncbi gi num :

4557757

ncbi acc num :

NP_000240.1

ncbi gb acc num :

NM_000249.3

uniprot acc num :

P40692

ncbi mol weight :

85 KD

ncbi pathways :

BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Direct P53 Effectors Pathway (137939); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Meiosis Pathway (477133); Meiotic Recombination Pathway (205242)

ncbi summary :

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

uniprot summary :

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage