product summary
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company name :
MyBioSource
product type :
antibody
product name :
TMEM30B Antibody
catalog :
MBS9604996
quantity :
0.1 mL
price :
240 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, enzyme immunoassay
more info or order :
image
image 1 :
MyBioSource MBS9604996 image 1
MBS9604996 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
image 2 :
MyBioSource MBS9604996 image 2
Western blot analysis of extracts from K562 cells, using TMEM30B antibody.
product information
catalog number :
MBS9604996
products type :
Antibody
products full name :
TMEM30B Antibody
products short name :
[TMEM30B]
products name syn :
[9130011B11Rik; CC50B_HUMAN; CDC50, S. cerevisiae, homolog of, B; CDC50B; Cell cycle control protein 50B; MGC126775; TMEM30B; Transmembrane protein 30B]
other names :
[cell cycle control protein 50B; Cell cycle control protein 50B; cell cycle control protein 50B; transmembrane protein 30B; P4-ATPase flippase complex beta subunit TMEM30B; Transmembrane protein 30B]
products gene name :
[TMEM30B]
other gene names :
[TMEM30B; TMEM30B; CDC50B; CDC50B]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse
sequence length :
351
specificity :
TMEM30B antibody detects endogenous levels of total TMEM30B
purity :
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
form :
Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
storage stability :
Store at -20 degree C. Stable for 12 months from date of receipt.
tested application :
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
app notes :
WB: 1:500-1:1000. IF/ICC: 1:100-1:500
image1 heading :
Immunofluorescence (IF)
image2 heading :
Western Blot (WB)
other info1 :
Immunogen: A synthesized peptide derived from human TMEM30B, corresponding to a region within the internal amino acids. Similarity: Belongs to the CDC50/LEM3 Family.
other info2 :
Important: For Western Blot (WB), incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween(R)20 at 4 degree C with gentle shaking, overnight.
products description :
Function: Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate (Probable). Can mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4 from the ER to the plasma membrane. Subunit Structure: Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (Probable). Interacts with alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4. Similarity: Belongs to the CDC50/LEM3 family.
ncbi gi num :
63003930
ncbi acc num :
NP_001017970.1
ncbi gb acc num :
NM_001017970.2
uniprot acc num :
Q3MIR4
ncbi mol weight :
38 kD
uniprot summary :
Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate (Probable). Can mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4 from the ER to the plasma membrane.
size1 :
0.1 mL
price1 :
240 USD
size2 :
0.2 mL
price2 :
280
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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