TOP3B Antibody | MyBioSource MBS9604527 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TOP3B Antibody

catalog :

MBS9604527

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9604527 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

image 2 :

Western blot analysis of extracts from Mouse brain, using TOP3B Ab. The lane on the left was treated with blocking peptide.

product information

catalog number :

MBS9604527

products type :

Antibody

products full name :

TOP3B Antibody

products short name :

[TOP3B]

products name syn :

[DNA topoisomerase 3 beta 1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta 1; DNA topoisomerase III beta-1; FLJ39376; TOP 3B; TOP3B; TOP3B_HUMAN; TOP3B1; Topo III beta; Topoisomerase (DNA) III beta; Topoisomerase DNA III beta; Topoisomerase III beta]

other names :

[DNA topoisomerase 3-beta-1 isoform 1; DNA topoisomerase 3-beta-1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta; DNA topoisomerase III beta-1]

products gene name :

[TOP3B]

other gene names :

[TOP3B; TOP3B; TOP3B1; TOP3B1]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

specificity :

TOP3B antibody detects endogenous levels of total TOP3B

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:500-1:1000. IF/ICC: 1:100-1:500. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Immunofluorescence (IF)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.

products description :

Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity. Similarity: Belongs to the type IA topoisomerase family.

ncbi gi num :

530788288

ncbi acc num :

NP_001269041.1

ncbi gb acc num :

NM_001282112.1

uniprot acc num :

O95985

ncbi mol weight :

96 KD

ncbi pathways :

Bloom's Syndrome Complex Pathway (413477); Bloom's Syndrome Complex Pathway (890629); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Homologous Recombination Pathway (83046); Homologous Recombination Pathway (454)

ncbi summary :

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]

uniprot summary :

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (). Possesses negatively supercoiled DNA relaxing activity.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage