REV1 Antibody | MyBioSource MBS9604095 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

REV1 Antibody

catalog :

MBS9604095

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9604095 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

Western blot analysis of REV1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.

product information

catalog number :

MBS9604095

products type :

Antibody

products full name :

REV1 Antibody

products short name :

[REV1]

products name syn :

[AIBP 80; AIBP80; Alpha integrin binding protein 80; Alpha integrin-binding protein 80; DNA repair protein REV 1; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV 1 (yeast homolog) like; REV 1; REV 1 homolog; REV 1 like; REV 1 protein; REV 1L; REV1 (yeast homolog) like; REV1; REV1 homolog (S. cerevisiae); REV1 homolog; REV1 like (yeast); REV1 like; Rev1 like terminal deoxycytidyl transferase; REV1 protein; Rev1-like terminal deoxycytidyl transferase; REV1_HUMAN; REV1L]

other names :

[DNA repair protein REV1 isoform 2; DNA repair protein REV1; DNA repair protein REV1; REV1, DNA directed polymerase; Alpha integrin-binding protein 80; AIBP80; Rev1-like terminal deoxycytidyl transferase]

products gene name :

[REV1]

other gene names :

[REV1; REV1; REV1L; AIBP80; REV1L; AIBP80]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1250

specificity :

REV1 antibody detects endogenous levels of total REV1

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Liquid. Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Predicted Cross Reactivity : Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus . Similarity: Pig (100%), Zebrafish (90%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)

other info2 :

Conjugation: Unconjugated

products description :

Description: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Subunit Structure: Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86. Similarity: The C-terminal domain is necessary for protein interactions. Belongs to the DNA polymerase type-Y family.

ncbi gi num :

84043967

ncbi acc num :

NP_001032961.1

ncbi gb acc num :

NM_001037872.2

uniprot acc num :

Q9UBZ9

ncbi mol weight :

Observed: 138 kDa. Predicted: 139 kDa

ncbi pathways :

DNA Damage Bypass Pathway (105852); DNA Repair Pathway (105837); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Translesion Synthesis By DNA Polymerases Bypassing Lesion On DNA Template Pathway (105853); Translesion Synthesis By HREV1 Pathway (105854)

ncbi summary :

This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]

uniprot summary :

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage