CACNA1C Antibody | MyBioSource MBS9603803 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CACNA1C Antibody

catalog :

MBS9603803

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of CACNA1C expression in Human Heart lysate in RIPA buffer, The lane on the left is treated with the antigen-specific peptide.

product information

catalog number :

MBS9603803

products type :

Antibody

products full name :

CACNA1C Antibody

products short name :

[CACNA1C]

other names :

[voltage-dependent L-type calcium channel subunit alpha-1C isoform 18; Voltage-dependent L-type calcium channel subunit alpha-1C; voltage-dependent L-type calcium channel subunit alpha-1C; calcium voltage-gated channel subunit alpha1 C; Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; Voltage-gated calcium channel subunit alpha Cav1.2]

products gene name :

[CACNA1C]

other gene names :

[CACNA1C; CACNA1C; TS; LQT8; CACH2; CACN2; CaV1.2; CCHL1A1; CACNL1A1; CACH2; CACN2; CACNL1A1; CCHL1A1]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

2138

specificity :

CACNA1C antibody detects endogenous levels of total CACNA1C.

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20°C. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB: 1:500-1:2000 - For Western Blots, incubate the membrane with diluted Ab in 5% w/v milk, 1X TBS , 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human CACNA1C, corresponding to a region within C-terminal amino acids.

products description :

Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death (PubMed:29078335). The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in opposite effects on the channel function. Similarity: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.

ncbi gi num :

120433602

ncbi acc num :

NP_000710.5

ncbi gb acc num :

NM_000719.6

uniprot acc num :

Q13936

ncbi mol weight :

249 kDa

ncbi pathways :

Adrenaline,noradrenaline Inhibits Insulin Secretion Pathway (1270103); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Aldosterone Synthesis And Secretion Pathway (1272485); Aldosterone Synthesis And Secretion Pathway (1285075); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546)

ncbi summary :

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012]

uniprot summary :

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:8392192, PubMed:7737988, PubMed:9087614, PubMed:9013606, PubMed:9607315, PubMed:12176756, PubMed:17071743, PubMed:11741969, PubMed:8099908, PubMed:12181424, PubMed:29078335, PubMed:29742403, PubMed:16299511, PubMed:20953164, PubMed:15454078, PubMed:15863612, PubMed:17224476, PubMed:24728418, PubMed:26253506, PubMed:27218670). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:15454078, PubMed:15863612, PubMed:17224476, PubMed:24728418, PubMed:26253506). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage