DSS1 Antibody | MyBioSource MBS9603380 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

DSS1 Antibody

catalog :

MBS9603380

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from B16F10 cells, using DSS1 Ab. The lane on the left was treated with blocking peptide.

image 2 :

Western blot analysis of extracts from HeLa cells, using DSS1 Ab.

image 3 :

MBS9603380 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

product information

catalog number :

MBS9603380

products type :

Antibody

products full name :

DSS1 Antibody

products short name :

[DSS1]

products name syn :

[26S proteasome complex subunit DSS1; C7orf76; Deleted in split hand/split foot 1; Deleted in split hand/split foot protein 1; Deleted in Split-Hand/Split-Foot 1 region; ECD; SEM1 26S proteasome complex subuni; SEM1; SHFD1; Shfdg1; SHFM1; SHSF1; Split hand/foot deleted protein 1; Split hand/foot malformation (ectrodactyly) type 1; Split hand/foot malformation type 1 protein]

other names :

[26S proteasome complex subunit SEM1 isoform 3; 26S proteasome complex subunit SEM1; 26S proteasome complex subunit SEM1; SEM1, 26S proteasome complex subunit; 26S proteasome complex subunit DSS1; Deleted in split hand/split foot protein 1; Split hand/foot deleted protein 1; Split hand/foot malformation type 1 protein]

products gene name :

[DSS1]

products gene name syn :

[SEM1]

other gene names :

[SEM1; SEM1; ECD; DSS1; SHFD1; SHFM1; SHSF1; Shfdg1; C7orf76]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

specificity :

DSS1 antibody detects endogenous levels of DSS1.

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Rabbit IgG in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Western Western (WB)

image3 heading :

Immunohistochemistry

other info1 :

Immunogen: A synthesized peptide derived from human DSS1. Corresponding to a region within the internal amino acids. Tissue Specificity: Expressed in limb bud, craniofacial primordia and skin. Predicted Cross Reactivity : Pig, Zebrafish, Bovine, Chicken, Xenopus . Similarity: Pig (100%), Zebrafish (100%), Bovine (100%), Chicken (100%), Xenopus (100%)

other info2 :

Conjugation: Unconjugated

products description :

Function: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:15117943). Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells. Subunit Structure: Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including SEM1, a base containing 6 ATPases and few additional components (PubMed:27428775, PubMed:27342858). Belongs to the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). Interacts with the C-terminal of BRCA2 (PubMed:10373512, PubMed:21719596). Similarity: Belongs to the DSS1/SEM1 family.

ncbi gi num :

5453640

ncbi acc num :

NP_006295.1

ncbi gb acc num :

NM_006304.1

uniprot acc num :

P60896

ncbi mol weight :

8-10 kDa

ncbi pathways :

Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Homologous Recombination Pathway (83046); Homologous Recombination Pathway (454); Proteasome Pathway (83040); Proteasome Pathway (445); Proteasome, 19S Regulatory Particle (PA700) Pathway (413447); Proteasome, 19S Regulatory Particle (PA700) Pathway (890593)

ncbi summary :

The product of this gene has been localized within the split hand/split foot malformation locus SHFM1 at chromosome 7. It has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. [provided by RefSeq, Jul 2008]

uniprot summary :

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed:15117943). Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage