ADAM12 Antibody | MyBioSource MBS9603245 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADAM12 Antibody

catalog :

MBS9603245

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9603245 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

image 2 :

Western blot analysis of extracts from Mouse liver, using ADAM12 Ab. The lane on the left was treated with blocking peptide.

product information

catalog number :

MBS9603245

products type :

Antibody

products full name :

ADAM12 Antibody

products short name :

[ADAM12]

products name syn :

[A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; CAR10; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; Meltrin alpha; Meltrin-alpha; Metalloprotease disintegrin 12 transmembrane; MLTN; MLTNA; OTTHUMP00000046766]

other names :

[disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein; Disintegrin and metalloproteinase domain-containing protein 12; disintegrin and metalloproteinase domain-containing protein 12; ADAM metallopeptidase domain 12; Meltrin-alpha]

products gene name :

[ADAM12]

other gene names :

[ADAM12; ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1; MLTN; ADAM 12]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

737

specificity :

ADAM12 antibody detects endogenous levels of ADAM12.

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human ADAM12.

products description :

Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity). Similarity: The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

ncbi gi num :

572882491

ncbi acc num :

NP_001275903.1

ncbi gb acc num :

NM_001288974.1

uniprot acc num :

O43184

ncbi mol weight :

97 kDa; 68 kDa

ncbi pathways :

Disease Pathway (530764); Notch Signaling Pathway (169345); Signal Transduction Pathway (477114); Signaling By EGFR Pathway (106337); Signaling By EGFR In Cancer Pathway (530768); Signaling By EGFRvIII In Cancer Pathway (1127577); Signaling By Ligand-Responsive EGFR Variants In Cancer Pathway (1127576); Signaling By Overexpressed Wild-Type EGFR In Cancer Pathway (1127579); Syndecan-4-mediated Signaling Events Pathway (138064)

ncbi summary :

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

uniprot summary :

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors ().

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage