PTRF Antibody | MyBioSource MBS9602917 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PTRF Antibody

catalog :

MBS9602917

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS9602917 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9602917 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.

image 2 :

Western blot analysis of extracts from mouse brain, using PTRF Ab.

product information

catalog number :

MBS9602917

products type :

Antibody

products full name :

PTRF Antibody

products short name :

[PTRF]

products name syn :

[2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12]

other names :

[caveolae-associated protein 1; Caveolae-associated protein 1; caveolae-associated protein 1; caveolae associated protein 1; Cavin-1; Polymerase I and transcript release factor]

products gene name :

[PTRF]

products gene name syn :

[CAVIN1]

other gene names :

[CAVIN1; CAVIN1; CGL4; PTRF; CAVIN; FKSG13; cavin-1; PTRF]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

390

specificity :

PTRF antibody detects endogenous levels of total PTRF

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

app notes :

WB: 1:1000-3000, IF/ICC 1:100-1:500. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Immunofluorescence/Immunocytochemistry (IF/ICC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human PTRF, corresponding to a region within the internal amino acids.

products description :

Function: Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed:18056712, PubMed:18191225, PubMed:19726876). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). Similarity: The leucine-zipper domain 1 is essential for its localization in the caveolae. Belongs to the CAVIN family.

ncbi gi num :

42734430

ncbi acc num :

NP_036364.2

ncbi gb acc num :

NM_012232.5

uniprot acc num :

Q6NZI2

ncbi mol weight :

43 kDa

ncbi pathways :

Gene Expression Pathway (105937); RNA Polymerase I Transcription Pathway (106550); RNA Polymerase I Transcription Termination Pathway (106556); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948)

ncbi summary :

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

uniprot summary :

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed:18056712, PubMed:18191225, PubMed:19726876). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template ().

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage