MMP1 Antibody | MyBioSource MBS9602703 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MMP1 Antibody

catalog :

MBS9602703

quantity :

0.05 mL

price :

235 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Figure 1: Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.

image 2 :

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using NFKB1 mouse mAb with DAB staining.

image 3 :

Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. / Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

product information

catalog number :

MBS9602703

products type :

Antibody

products full name :

MMP1 Antibody

products short name :

[MMP1]

other names :

[interstitial collagenase isoform 1 preproprotein; Interstitial collagenase; interstitial collagenase; matrix metallopeptidase 1; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1]

products gene name :

[MMP1]

other gene names :

[MMP1; MMP1; CLG; CLGN; CLG; MMP-1]

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

reactivity :

Human

sequence length :

469

specificity :

MMP1 antibody detects endogenous levels of total MMP1.

purity :

Affinity-Chromatography

form :

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)

app notes :

ELISA: 1:10000. WB: 1:500-1:2000. IHC: 1:200-1:1000. ICC: 1:200-1:1000. FC/FACS: 1:200-1:400. IMPORTANT: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Purified recombinant fragment of human MMP1 expressed in E. Coli.

products description :

Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Belongs to the peptidase M10A family.

ncbi gi num :

4505215

ncbi acc num :

NP_002412.1

ncbi gb acc num :

NM_002421.3

uniprot acc num :

P03956

ncbi mol weight :

54 kDa

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Basigin Interactions Pathway (106065); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Surface Interactions At The Vascular Wall Pathway (106062); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (762258); Degradation Of The Extracellular Matrix Pathway (576263); Degradation Of The Extracellular Matrix Pathway (762256); Endothelins Pathway (137958)

ncbi summary :

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

uniprot summary :

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

295

size3 :

0.2 mL

price3 :

370

more info or order :

MyBioSource product webpage