Tyrosinase Antibody | MyBioSource MBS9601658 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Tyrosinase Antibody

catalog :

MBS9601658

quantity :

0.1 mL

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot (WB) analysis of extracts from various samples, using Tyrosinase Ab. Lane 1: Hela cells, treated with blocking peptide;. Lane 2: Hela cells;. Lane 3: COS-7;. Lane 4: B16F10 cells.

image 2 :

Western blot analysis of extracts from HUVEC, using Tyrosinase Ab. The lane on the left was treated with blocking peptide.

image 3 :

Western blot analysis of Tyrosinase expression in COS7 cells.The lane on the left was treated with the antigen-specific peptide.

product information

catalog number :

MBS9601658

products type :

Antibody

products full name :

Tyrosinase Antibody

products short name :

[Tyrosinase]

products name syn :

[ATN; CMM8; LB24 AB; LB24-AB; Monophenol monooxygenase; OCA1; OCA1A; OCAIA; Oculocutaneous albinism IA; SHEP3; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; TYRO_HUMAN; tyrosinase (oculocutaneous albinism IA); Tyrosinase]

other names :

[tyrosinase; Tyrosinase; tyrosinase; tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB]

products gene name :

[TYR]

other gene names :

[TYR; TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Monkey

sequence length :

529

specificity :

Tyrosinase antibody detects endogenous levels of total Tyrosinase

purity :

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

form :

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C.Stable for 12 months from date of receipt.

tested application :

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IF: 1:100-1:500

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

MBS9601658 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.

image5 heading :

Immunofluorescence (IF)

image5 description :

MBS9601658 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9601658 1:200) and mouse antibeta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.

other info1 :

Immunogen: A synthesized peptide derived from human Tyrosinase, corresponding to a region within C-terminal amino acids. Subcellular Location: Melanosome membrane. Tissue Specificity: Increased expression after UVB irradiation. Similarity: Belongs to the tyrosinase family.

other info2 :

Importatnt: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

products description :

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.

ncbi gi num :

4507753

ncbi acc num :

NP_000363.1

ncbi gb acc num :

NM_000372.4

uniprot acc num :

P14679

ncbi mol weight :

70 kDa

ncbi pathways :

(S)-reticuline Biosynthesis II Pathway (547499); (S)-reticuline Biosynthesis II Pathway (138845); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); L-dopachrome Biosynthesis Pathway (545322); L-dopachrome Biosynthesis Pathway (545496); Melanogenesis Pathway (83092); Melanogenesis Pathway (504); Riboflavin Metabolism Pathway (83012); Riboflavin Metabolism Pathway (398)

ncbi summary :

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]

uniprot summary :

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.

size1 :

0.1 mL

price1 :

240 USD

size2 :

0.2 mL

price2 :

280

more info or order :

MyBioSource product webpage