product summary
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company name :
MyBioSource
product type :
antibody
product name :
FZD2 Antibody
catalog :
MBS9601455
quantity :
0.1 mL
price :
240 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunocytochemistry, enzyme immunoassay
more info or order :
image
image 1 :
MBS9601455 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
image 2 :
Western blot analysis of FZD2 expression in HeLa cells
product information
catalog number :
MBS9601455
products type :
Antibody
products full name :
FZD2 Antibody
products short name :
[FZD2]
products name syn :
[Frizzled homolog 2; Frizzled-2; Frizzled2; Fz 2; Fz-2; Fz2; FZD 2; FZD2; FZD2_HUMAN; FzE 2; FzE2; hFz 2; hFz2; Mfz10]
other names :
[frizzled-2; Frizzled-2; frizzled-2; frizzled class receptor 2; FzE2]
products gene name :
[FZD2]
other gene names :
[FZD2; FZD2; Fz2; fz-2; fzE2; hFz2; Fz-2; hFz2]
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human, Mouse, Rat
sequence length :
565
specificity :
FZD2 antibody detects endogenous levels of total FZD2
purity :
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
form :
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
concentration :
1mg/ml
storage stability :
Store at -20 degree C. Stable for 12 months from date of receipt.
tested application :
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
app notes :
WB: 1:500-1:2000. IF: 1:100-1:500
image1 heading :
Immunofluorescence (IF)
image2 heading :
Western Blot (WB)
other info1 :
Immunogen: A synthesized peptide derived from human FZD2, corresponding to a region within the internal amino acids.
other info2 :
Similarity: Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.The FZ domain is involved in binding with Wnt ligands. Belongs to the G-protein coupled receptor Fz/Smo family.
ncbi gi num :
4503827
ncbi acc num :
NP_001457.1
ncbi gb acc num :
NM_001466.3
uniprot acc num :
Q14332
ncbi mol weight :
63 kDa
ncbi pathways :
Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)
ncbi summary :
This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]
uniprot summary :
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
size1 :
0.1 mL
price1 :
240 USD
size2 :
0.2 mL
price2 :
280
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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