protein

Recombinant Human Vinculin

MBS957842

0.05 mg (E-Coli)

165 USD

Recombinant Protein

Recombinant Human Vinculin

Vinculin

Metavinculin; MV

vinculin isoform VCL; Vinculin; vinculin; vinculin; Metavinculin; MV

VCL

VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV

VINC_HUMAN

E Coli

2-235

1066

PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDL
TAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFI
KVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSG
TSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDL
VTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMN
TVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVE

Greater than 90% as determined by SDS-PAGE.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Cell Adhesion

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Complete sequence of human vinculin and assignment of the gene to chromosome 10.Weller P.A., Ogryzko E.P., Corben E.B., Zhidkova N.I., Patel B., Price G.J., Spurr N.K., Koteliansky V.E., Critchley D.R.Proc. Natl. Acad. Sci. U.S.A. 87:5667-5671(1990) The full-ORF clone resource of the German cDNA consortium.Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.BMC Genomics 8:399-399(2007) The DNA sequence and comparative analysis of human chromosome 10.Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.Nature 429:375-381(2004) Organization of the human gene encoding the cytoskeletal protein vinculin and the sequence of the vinculin promoter.Moiseyeva E.P., Weller P.A., Zhidkova N.I., Corben E.B., Patel B., Jasinska I., Koteliansky V.E., Critchley D.R.J. Biol. Chem. 268:4318-4325(1993) Lubec G., Chen W.-Q., Sun Y.Submitted (DEC-2008) to UniProtKB An additional exon in the human vinculin gene specifically encodes meta-vinculin-specific difference peptide. Cross-species comparison reveals variable and conserved motifs in the meta-vinculin insert.Koteliansky V.E., Ogryzko E.P., Zhidkova N.I., Weller P.A., Critchley D.R., Vancompernolle K., Vandekerckhove J., Strasser P., Way M., Gimona M., Small J.V.Eur. J. Biochem. 204:767-772(1992) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.Nat. Biotechnol. 21:566-569(2003) The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading.Zhang Z., Izaguirre G., Lin S.-Y., Lee H.Y., Schaefer E., Haimovich B.Mol. Biol. Cell 15:4234-4247(2004) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.Cell 127:635-648(2006) Human alpha-synemin interacts directly with vinculin and metavinculin.Sun N., Critchley D.R., Paulin D., Li Z., Robson R.M.Biochem. J. 409:657-667(2008) Cell cycle regulated phosphorylation of LIMD1 in cell lines and expression in human breast cancers.Huggins C.J., Andrulis I.L.Cancer Lett. 267:55-66(2008) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) Lysine acetylation targets protein complexes and co-regulates major cellular functions.Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2009) Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein.Le Clainche C., Dwivedi S.P., Didry D., Carlier M.F.J. Biol. Chem. 285:23420-23432(2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.Sci. Signal. 3:RA3-RA3(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011) The C-terminal tail domain of metavinculin, vinculin's splice variant, severs actin filaments.Janssen M.E., Liu H., Volkmann N., Hanein D.J. Cell Biol. 197:585-593(2012) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) Structural basis for amplifying vinculin activation by talin.Izard T., Vonrhein C.J. Biol. Chem. 279:27667-27678(2004) Vinculin activation by talin through helical bundle conversion.Izard T., Evans G., Borgon R.A., Rush C.L., Bricogne G., Bois P.R.J.Nature 427:171-175(2004) Metavinculin mutations alter actin interaction in dilated cardiomyopathy.Olson T.M., Illenberger S., Kishimoto N.Y., Huttelmaier S., Keating M.T., Jockusch B.M.Circulation 105:431-437(2002) Identification of a metavinculin missense mutation, R975W, associated with both hypertrophic and dilated cardiomyopathy.Vasile V.C., Will M.L., Ommen S.R., Edwards W.D., Olson T.M., Ackerman M.J.Mol. Genet. Metab. 87:169-174(2006) A missense mutation in a ubiquitously expressed protein, vinculin, confers susceptibility to hypertrophic cardiomyopathy.Vasile V.C., Ommen S.R., Edwards W.D., Ackerman M.J.Biochem. Biophys. Res. Commun. 345:998-1003(2006)

4507877

NP_003364.1

NM_003373.3

P18206

53.4kD

ARMS-mediated Activation Pathway (1269471); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Axon Guidance Pathway (1270303); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283)

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: actin cytoskeleton; adherens junction; brush border; cell; cell-cell adherens junction; cell-matrix junction; costamere; cytoskeleton; cytosol; extracellular region; fascia adherens; focal adhesion; intercellular junction; lipid raft; plasma membrane; protein complex; zonula adherens. Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; structural molecule activity; ubiquitin protein ligase binding. Biological Process: apical junction assembly; axon extension; blood coagulation; cell adhesion; cell motility; cell-matrix adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; platelet activation; platelet degranulation. Disease: Cardiomyopathy, Dilated, 1w

0.05 mg (E-Coli)

165 USD

0.2 mg (E-Coli)

275

0.5 mg (E-Coli)

515

1 mg (E-Coli)

755