protein

Recombinant Human Serine/threonine-protein kinase pim-1 (PIM1)

MBS957553

0.5 mg (E-Coli)

1175 USD

Recombinant Protein

Recombinant Human Serine/threonine-protein kinase pim-1 (PIM1)

Serine/threonine-protein kinase pim-1 (PIM1)

Serine/threonine-protein kinase pim-1; EC=2.7.11.1

serine/threonine-protein kinase pim-1 isoform 1; Serine/threonine-protein kinase pim-1; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; Pim-1 proto-oncogene, serine/threonine kinase

PIM1

PIM1; PIM1; PIM

PIM1_HUMAN

E Coli or Yeast or Baculovirus or Mammalian Cell

1-404

404

MPHEPHEPLT PPFSALPDPA GAPSRRQSRQ RPQLSSDSPS AFRASRSHSR NATRSHSHSH SPRHSLRHSP GSGSCGSSSG HRPCADILEV GMLLSKINSL AHLRAAPCND LHATKLAPGK EKEPLESQYQ VGPLLGSGGF GSVYSGIRVS DNLPVAIKHV EKDRISDWGE LPNGTRVPME VVLLKKVSSG FSGVIRLLDW FERPDSFVLI LERPEPVQDL FDFITERGAL QEELARSFFW QVLEAVRHCH NCGVLHRDIK DENILIDLNR GELKLIDFGS GALLKDTVYT DFDGTRVYSP PEWIRYHRYH GRSAAVWSLG ILLYDMVCGD IPFEHDEEII RGQVFFRQRV SSECQHLIRW CLALRPSDRP TFEEIQNHPW MQDVLLPQET AEIHLHSLSP GPSK

>90%

Liquid containing glycerol; lyophilization may be available upon request.

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Species: Homo sapiens (Human)

342307046

NP_001230115.1

NM_001243186.1

P11309

35,686 Da

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); C-MYB Transcription Factor Network Pathway (138073); IL-5 Signaling Pathway (198770); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway (137953); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]

Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines. Protein type: Protein kinase, CAMK; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; PIM family. Chromosomal Location of Human Ortholog: 6p21.2. Cellular Component: cytoplasm; plasma membrane; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; manganese ion binding; transcription factor binding; ATP binding; ribosomal small subunit binding. Biological Process: cell proliferation; apoptosis; negative regulation of transcription factor activity; multicellular organismal development; protein amino acid autophosphorylation; regulation of mitotic cell cycle; cell cycle; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; hyaluronan metabolic process; negative regulation of apoptosis

0.5 mg (E-Coli)

1175 USD

0.05 mg (Baculovirus)

1175

0.5 mg (Yeast)

1395

0.05 mg (Mammalian-Cell)

1395

1 mg (E-Coli)

1890