protein

Recombinant Human E3 SUMO-protein ligase RanBP2 (RANBP2), partial

MBS951299

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Recombinant Protein

Recombinant Human E3 SUMO-protein ligase RanBP2 (RANBP2), partial

E3 SUMO-protein ligase RanBP2 (RANBP2), partial

E3 SUMO-protein ligase RanBP2; EC=6.3.2.-; 358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270; Including the following 1 domains:; Putative peptidyl-prolyl cis-trans isomerase; PPIase; EC=5.2.

E3 SUMO-protein ligase RanBP2; E3 SUMO-protein ligase RanBP2; E3 SUMO-protein ligase RanBP2; P270; nucleoporin 358; nucleoporin Nup358; 358 kDa nucleoporin; ran-binding protein 2; transformation-related protein 2; nuclear pore complex protein Nup358; acute necrotizing encephalopathy 1 (autosomal dominant); RAN binding protein 2; 358 kDa nucleoporin; Nuclear pore complex protein Nup358; Nucleoporin Nup358; Ran-binding protein 2; RanBP2; p270

RANBP2

RANBP2; NUP358

RANBP2; RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358; NUP358; RanBP2

RBP2_HUMAN

E Coli or Yeast or Baculovirus or Mammalian Cell

>90%

Liquid containing glycerol; lyophilization may be available upon request.

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Species: Homo sapiens (Human)

E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Binds single-stranded RNA (in vitro). May bind DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB.

150418007

NP_006258.3

NM_006267.4

P49792

358,199 Da

Cell Cycle, Mitotic Pathway (105765); DNA Replication Pathway (105897); Export Of Viral Ribonucleoproteins From Nucleus Pathway (106080); Formation And Maturation Of MRNA Transcript Pathway (105940); Gene Expression Pathway (105937); Glucose Transport Pathway (106200); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Hexose Transport Pathway (106198); Host Interactions Of HIV Factors Pathway (106010)

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008]

RANBP2: a very large RAN-binding nucleoporin with E3 SUMO-protein ligase activity. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Forms a complex with SUMOylated RanGAP1 (the GTPase activating protein for Ran) that is required for efficient importin alpha/beta dependent nuclear import. May bind RNA or DNA. Component of the nuclear export pathway. Has a specific docking site for the nuclear export factor exportin-1. Contains a cyclophilin type peptidyl-prolyl cis-trans isomerase domain. A giant scaffolding protein that forms filaments extending from the nuclear pore into the cytoplasm. Regulates the localization of mitochondria in photosensory neurons. Deficits of RanBP2 downregulate glucose catabolism and energy homeostasis. Forms a tight complex with RANBP1 and UBE2I. Interacts with SUMO1 and PARK2. For some substrates, sumoylation and nuclear import are linked events. Sumoylates proteins including SP100 and HDAC4 deacetylase. Protein type: EC 6.3.2.-; Ligase; Adaptor/scaffold; Nuclear export; EC 5.2.1.8; SUMO conjugating system; Nucleoporin. Chromosomal Location of Human Ortholog: 2q12.3. Cellular Component: nuclear membrane; mitochondrion; membrane; nuclear pore; cytosol; nuclear inclusion body. Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; zinc ion binding; RNA binding; Ran GTPase binding; ligase activity. Biological Process: mRNA transport; protein peptidyl-prolyl isomerization; viral reproduction; protein folding; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; negative regulation of glucokinase activity; protein sumoylation; protein import into nucleus; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport. Disease: Encephalopathy, Acute, Infection-induced, Susceptibility To, 3

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