protein

Recombinant Mouse AP-2 complex subunit mu (Ap2m1)

MBS950549

0.5 mg (E-Coli)

1210 USD

Recombinant Protein

Recombinant Mouse AP-2 complex subunit mu (Ap2m1)

AP-2 complex subunit mu (Ap2m1)

AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 mu subunit; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu

AP-2 complex subunit mu; AP-2 complex subunit mu; AP-2 complex subunit mu; mu2-adaptin; AP-2 mu chain; clathrin-associated AP-2; adaptor protein complex AP-2, mu1; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50; adaptor protein complex AP-2 subunit mu; adaptor-related protein complex AP-2, mu1; plasma membrane adaptor AP-2 50 kDa protein; adapter-related protein complex 2 mu subunit; adapter-related protein complex 2 subunit mu; adaptor-related protein complex 2 subunit mu; clathrin assembly protein complex 2 medium chain; clathrin assembly protein complex 2 mu medium chain; adaptor-related protein complex 2, mu 1 subunit; AP-2 mu chain; Adaptor protein complex AP-2 subunit mu; Adaptor-related protein complex 2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein

Ap2m1

Ap2m1; Clapm1

Ap2m1; Ap2m1; Clapm1

AP2M1_MOUSE

E Coli or Yeast or Baculovirus or Mammalian Cell

1-435

435

MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI ARTSFFHVKR SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN IKNNFVLIYE LLDEILDFGY PQNSETGALK TFITQQGIKS QHQTKEEQSQ ITSQVTGQIG WRREGIKYRR NELFLDVLES VNLLMSPQGQ VLSAHVSGRV VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS IAIDDCTFHQ CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA IVWKIKRMAG MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK VRYLKVFEPK LNYSDHDVIK WVRYIGRSGI YETRC

>90%

Liquid containing glycerol; lyophilization may be available upon request.

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Species: Mus musculus (Mouse)

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 . Plays a role in endocytosis of frizzled family members upon Wnt signaling

6753074

NP_033809.1

NM_009679.2

P84091

49,655 Da

Adaptive Immune System Pathway (971210); Axon Guidance Pathway (970585); Developmental Biology Pathway (970813); Disease Pathway (970606); EGFR Downregulation Pathway (1000610); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213309); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Formation Of Annular Gap Junctions Pathway (1000305)

This gene encodes the mu subunit of the clathrin adaptor protein complex AP-2. It mediates sorting of cargo proteins harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate splicing of this gene results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2, 8 and 19. [provided by RefSeq, Dec 2014]

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17). Protein type: Vesicle; Adaptor/scaffold. Cellular Component: clathrin adaptor complex; mitochondrion; membrane; plasma membrane; terminal button; coated pit; secretory granule. Molecular Function: low-density lipoprotein receptor binding; signal sequence binding; lipid binding. Biological Process: vesicle-mediated transport; intracellular protein transport; protein transport; transport; endocytosis

0.5 mg (E-Coli)

1210 USD

0.05 mg (Baculovirus)

1210

0.5 mg (Yeast)

1430

0.05 mg (Mammalian-Cell)

1430

1 mg (E-Coli)

1960