ELISA/assay

IL-1beta (Rat) OmniKine ELISA Kit

MBS9501933

96-Strip-Wells

440 USD

ELISA Kit

IL-1beta (Rat) OmniKine ELISA Kit

[IL-1beta]

[Interleukin-1 beta; IL-1 beta]

[Interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; interleukin 1 beta]

[IL-1beta]

[Il1b]

[Il1b; Il1b; IL-1F2; IL-1 beta]

Rat

268

The OmniKine Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.

12 x 8-Well Microstrips

Store at 4 degree C for 6 months.

Testing Data

Assay Type: Quantitative Sandwich. Sensitivity: 63-4000 pg/ml.

Principle of the Assay: The OmniKine Murine JE/MCP-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine JE/MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine JE/MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine JE/MCP-1 or Monocyte Chemotactic Protein 1, mainly known as C-C Motif Chemokine 2, is a 148 amino acid cytokine protein expressed from chromosome 11. After initial synthesis and translocation, the 23 residue signal sequence is cleaved, allowing the 125 residue JE/MCP-1 peptide to properly fold and mature. Belonging to the intercrine beta (chemokine C-C) family, the monomeric and homodimeric JE/MCP-1 proteins function as chemotactic factors that attract monocytes, but not neutrophils. Furthermore, they are typically induced by platelet-derived growth factors (PDGF).

2497335

Q63264.1

Q63264

30,644 Da

African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Apoptosis Pathway (83452); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795)

an inflammatory cytokine [RGD, Feb 2006]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 3q36. Cellular Component: autophagic vacuole; cytoplasmic vesicle; cytosol; extracellular region; extracellular space; lysosome; secretory granule; vesicle. Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding. Biological Process: activation of MAPK activity; acute inflammatory response; chronic inflammatory response to antigenic stimulus; cytokine-mediated signaling pathway; elevation of cytosolic calcium ion concentration; fever; germ cell programmed cell death; hyaluronan biosynthetic process; immune response; inflammatory response; interleukin-1 beta production; interleukin-6 production; learning and/or memory; leukocyte migration; lipopolysaccharide-mediated signaling pathway; MAPK cascade; memory; negative regulation of cell proliferation; negative regulation of glutamate secretion; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of synaptic transmission; negative regulation of transcription from RNA polymerase II promoter; neutrophil chemotaxis; ovulation; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of fever; positive regulation of glial cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of immature T cell proliferation in the thymus; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitotic nuclear division; positive regulation of neuron apoptotic process; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein kinase B signaling; regulation of defense response to virus by host; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; response to ATP; response to carbohydrate stimulus; response to drug; response to estradiol; response to ethanol; response to gamma radiation; response to glucocorticoid stimulus; response to heat; response to hypoxia; response to L-ascorbic acid; response to lipopolysaccharide; response to morphine; response to nutrient; response to organic cyclic compound; response to organonitrogen compound; response to ozone; response to peptide hormone; response to vitamin D; sequestering of triacylglycerol; social behavior; wound healing

96-Strip-Wells

440 USD