protein

Recombinant Human Endothelin-1 protein

MBS949552

0.05 mg (E-Coli)

190 USD

Recombinant Protein

Recombinant Human Endothelin-1 protein

Endothelin-1 protein

Preproendothelin-1; PPET1

endothelin-1 isoform 2 preproprotein; Endothelin-1; endothelin-1; endothelin 1; Preproendothelin-1; PPET1

EDN1

EDN1; EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7; PPET1; ET-1

EDN1_HUMAN

E Coli

53-73

211

CSCSSLMDKECVYFCHLDIIW

Greater than 90% as determined by SDS-PAGE.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Signal Transduction

Endothelins are endothelium-derived vasoconstrictor peptides.

Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin identity of human and porcine endothelin.Itoh Y., Yanagisawa M., Ohkubo S., Kimura C., Kosaka T., Inoue A., Ishida N., Mitsui Y., Onda H., Fujino M., Masaki T.FEBS Lett. 231:440-444(1988) The human preproendothelin-1 gene. Complete nucleotide sequence and regulation of expression.Inoue A., Yanagisawa M., Takuwa Y., Mitsui Y., Kobayashi M., Masaki T.J. Biol. Chem. 264:14954-14959(1989) Structural organization and chromosomal assignment of the gene encoding endothelin.Bloch K.D., Friedrich S.P., Lee M.E., Eddy R.L., Shows T.B., Quertermous T.J. Biol. Chem. 264:10851-10857(1989) Two preproendothelin 1 mRNAs transcribed by alternative promoters.Benatti L., Bonecchi L., Cozzi L., Sarmientos P.J. Clin. Invest. 91:1149-1156(1993) NIEHS SNPs programThe DNA sequence and analysis of human chromosome 6.Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.Nature 425:805-811(2003) The human endothelin family three structurally and pharmacologically distinct isopeptides predicted by three separate genes.Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T.Proc. Natl. Acad. Sci. U.S.A. 86:2863-2867(1989) Proteolytic processing of big endothelin-3 by the kell blood group protein.Lee S., Lin M., Mele A., Cao Y., Farmar J., Russo D., Redman C.Blood 94:1440-1450(1999) The signal peptide of human preproendothelin-1.Fabbrini M.S., Valsasina B., Nitti G., Benatti L., Vitale A.FEBS Lett. 286:91-94(1991) Endothelin-1 and ETA receptor expression in vascular smooth muscle cells from human placenta a new ETA receptor messenger ribonucleic acid is generated by alternative splicing of exon 3.Bourgeois C., Robert B., Rebourcet R., Mondon F., Mignot T.-M., Duc-Goiran P., Ferre F.J. Clin. Endocrinol. Metab. 82:3116-3123(1997) Genetic analysis of 103 candidate genes for coronary artery disease and associated phenotypes in a founder population reveals a new association between endothelin-1 and high-density lipoprotein cholesterol.Pare G., Serre D., Brisson D., Anand S.S., Montpetit A., Tremblay G., Engert J.C., Hudson T.J., Gaudet D.Am. J. Hum. Genet. 80:673-682(2007) Investigating the association between K198N coding polymorphism in EDN1 and hypertension, lipoprotein levels, the metabolic syndrome and cardiovascular disease.Wiltshire S., Powell B.L., Jennens M., McCaskie P.A., Carter K.W., Palmer L.J., Thompson P.L., McQuillan B.M., Hung J., Beilby J.P.Hum. Genet. 123:307-313(2008) Crystallization and preliminary X-ray analysis of human endothelin.Wolff M., Day J., Greenwood A., Larson S., McPherson A.Acta Crystallogr. B 48:239-240(1992) The crystal structure of human endothelin.Janes R.W., Peapus D.H., Wallace B.A.Nat. Struct. Biol. 1:311-319(1994) The conformation of endothelin-1 in aqueous solution NMR-derived constraints combined with distance geometry and molecular dynamics calculations.Reily M.D., Dunbar J.B. Jr.Biochem. Biophys. Res. Commun. 178:570-577(1991) Conformational isomerism of endothelin in acidic aqueous media a quantitative NOESY analysis.Andersen N.H., Chen C., Marschner T.M., Krystek S.R. Jr., Bassolino D.A.Biochemistry 31:1280-1295(1992) Solution conformation of human big endothelin-1.Donlan M.L., Brown F.K., Jeffs P.W.J. Biomol. NMR 2:407-420(1992) A comparison of X-ray and NMR structures for human endothelin-1.Wallace B.A., Janes R.W., Bassolino D.A., Krystek S.R. Jr.Protein Sci. 4:75-83(1995) Solution structure of a novel ETB receptor selective agonist ET1-21 by nuclear magnetic resonance spectroscopy and molecular modelling.Hewage C.M., Jiang L., Parkinson J.A., Ramage R., Sadler I.H.J. Pept. Res. 53:223-233(1999) The Lys198Asn polymorphism in the endothelin-1 gene is associated with blood pressure in overweight people.Tiret L., Poirier O., Hallet V., McDonagh T.A., Morrison C., McMurray J.J., Dargie H.J., Arveiler D., Ruidavets J.B., Luc G., Evans A., Cambien F.Hypertension 33:1169-1174(1999) Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis.Halushka M.K., Fan J.-B., Bentley K., Hsie L., Shen N., Weder A., Cooper R., Lipshutz R., Chakravarti A.Nat. Genet. 22:239-247(1999) Mutations in endothelin 1 cause recessive auriculocondylar syndrome and dominant isolated question-mark ears.Gordon C.T., Petit F., Kroisel P.M., Jakobsen L., Zechi-Ceide R.M., Oufadem M., Bole-Feysot C., Pruvost S., Masson C., Tores F., Hieu T., Nitschke P., Lindholm P., Pellerin P., Guion-Almeida M.L., Kokitsu-Nakata N.M., Vendramini-Pittoli S., Munnich A., Lyonnet S., Holder-Espinasse M., Amiel J.Am. J. Hum. Genet. 93:1118-1125(2013)

269995983

NP_001161791.1

NM_001168319.1

P05305

29.9kD

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); EGFR-dependent Endothelin Signaling Events Pathway (138037); Endothelin Pathway (672453); Endothelins Pathway (137958); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592)

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p24.1. Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space. Molecular Function: cytokine activity; endothelin A receptor binding; endothelin B receptor binding; hormone activity; protein binding. Biological Process: artery smooth muscle contraction; body fluid secretion; calcium-mediated signaling; cartilage development; cell growth; cell surface receptor linked signal transduction; cell-cell signaling; dorsal/ventral pattern formation; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); epithelial fluid transport; G-protein coupled receptor protein signaling pathway; G-protein signaling, phospholipase D activating pathway; glucose transport; heart development; histamine secretion; in utero embryonic development; inositol phosphate-mediated signaling; leukocyte activation; maternal process involved in parturition; membrane depolarization; middle ear morphogenesis; multicellular organismal aging; negative regulation of blood coagulation; negative regulation of cAMP biosynthetic process; negative regulation of cellular protein metabolic process; negative regulation of hormone secretion; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; neural crest cell development; neutrophil chemotaxis; nitric oxide transport; patterning of blood vessels; peptide hormone secretion; phosphoinositide 3-kinase cascade; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of heart rate; positive regulation of hormone secretion; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of nitric oxide biosynthetic process; positive regulation of odontogenesis; positive regulation of prostaglandin secretion; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of transcription from RNA polymerase II promoter; prostaglandin biosynthetic process; protein kinase C activation; protein kinase C deactivation; regulation of pH; regulation of sensory perception of pain; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; respiratory gaseous exchange; response to activity; response to amino acid stimulus; response to lipopolysaccharide; response to nicotine; response to ozone; response to testosterone stimulus; rhythmic excitation; sensory perception of pain; superoxide release; vasoconstriction; vein smooth muscle contraction. Disease: Auriculocondylar Syndrome 3; Question Mark Ears, Isolated

0.05 mg (E-Coli)

190 USD

0.2 mg (E-Coli)

460

0.5 mg (E-Coli)

750

1 mg (E-Coli)

1180