protein

Recombinant Human 40S ribosomal protein S3

MBS947991

0.01 mg (E-Coli)

135 USD

Recombinant Protein

Recombinant Human 40S ribosomal protein S3

40S ribosomal protein S3

Homo sapiens (Human)

40S ribosomal protein S3 isoform 1; 40S ribosomal protein S3; 40S ribosomal protein S3; ribosomal protein S3

RPS3

RPS3; RPS3; S3

RS3_HUMAN

E Coli or Yeast or Baculovirus or Mammalian Cell

2-243

243

AVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVR
VTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGF
PEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRA
CYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLM
IHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTG
KIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPA
MPQPVPTA

Greater than 90% as determined by SDS-PAGE.

Liquid containing glycerol; lyophilization may be available upon request.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Species: Homo sapiens (Human)

Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408).

Isolation of a cDNA encoding human 40S ribosomal protein s3." Zhang X.T., Tan Y.M., Tan Y.H. Nucleic Acids Res. 18:6689-6689(1990)

15718687

NP_000996.2

NM_001005.4

P23396

42.54kD

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (1268683); Cap-dependent Translation Initiation Pathway (1268680); Cytoplasmic Ribosomal Proteins Pathway (198853); Disease Pathway (1268854); Eukaryotic Translation Elongation Pathway (1268690); Eukaryotic Translation Initiation Pathway (1268679); Eukaryotic Translation Termination Pathway (1268692); Formation Of A Pool Of Free 40S Subunits Pathway (1268681); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (1268682); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (1268686)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer. Protein type: RNA-binding; EC 4.2.99.18; Apoptosis; Translation; Ribosomal. Chromosomal Location of Human Ortholog: 11q13.3-q13.5. Cellular Component: cytoplasm; cytosol; dendrite; endomembrane system; focal adhesion; membrane; mitochondrial inner membrane; mitochondrial matrix; nucleolus; nucleus; plasma membrane; polysome; ribonucleoprotein complex; ribosome. Molecular Function: damaged DNA binding; DNA binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; iron-sulfur cluster binding; kinase binding; microtubule binding; mRNA binding; NF-kappaB binding; oxidized purine base lesion DNA N-glycosylase activity; oxidized purine DNA binding; oxidized pyrimidine DNA binding; protein binding; protein kinase A binding; protein kinase binding; RNA binding; structural constituent of ribosome; SUMO binding; transcription factor binding; tubulin binding. Biological Process: activation of NF-kappaB transcription factor; cell division; cellular protein metabolic process; chromosome segregation; DNA damage response, detection of DNA damage; DNA repair; gene expression; mitosis; mRNA catabolic process, nonsense-mediated decay; negative regulation of DNA repair; negative regulation of protein ubiquitination; negative regulation of translation; positive regulation of DNA repair; positive regulation of endodeoxyribonuclease activity; positive regulation of JNK activity; positive regulation of microtubule polymerization; regulation of apoptosis; response to DNA damage stimulus; response to oxidative stress; selenium metabolic process; selenocysteine metabolic process; spindle assembly; SRP-dependent cotranslational protein targeting to membrane; transcription, DNA-dependent; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription

0.01 mg (E-Coli)

135 USD

0.05 mg (E-Coli)

255

0.2 mg (E-Coli)

665

0.05 mg (Baculovirus)

990

0.5 mg (E-Coli)

1090