Mouse Lipopolysaccharide-binding protein, LBP ELISA Kit | MyBioSource MBS947489 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Lipopolysaccharide-binding protein, LBP ELISA Kit

catalog :

MBS947489

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS947489

products type :

ELISA Kit

products full name :

Mouse Lipopolysaccharide-binding protein, LBP ELISA Kit

products short name :

lipopolysaccharide binding protein

products name syn :

Mouse Lipopolysaccharide-binding protein (LBP) ELISA kit; MGC22233; LPS-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein

other names :

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; lipopolysaccharide binding protein

products gene name :

LBP

other gene names :

Lbp; Lbp; Ly88; Bpifd2; LBP

uniprot entry name :

LBP_MOUSE

reactivity :

Mouse

sequence length :

481

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse LBP. No significant cross-reactivity or interference between mouse LBP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 ng/ml -800 ng/ml. Sensitivity: The minimum detectable dose of mouse LBP is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

113865991

ncbi acc num :

NP_032515.2

ncbi gb acc num :

NM_008489.2

uniprot acc num :

Q61805

ncbi mol weight :

53,055 Da

ncbi pathways :

Immune System Pathway (575095); Innate Immune System Pathway (575115); LPS Transferred From LBP Carrier To CD14 Pathway (575139); NF-kappa B Signaling Pathway (634537); Salmonella Infection Pathway (375174); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (575138); Toll Receptor Cascades Pathway (575116); Toll-like Receptor Signaling Pathway (83273); Toll-like Receptor Signaling Pathway (487)

uniprot summary :

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: lipopolysaccharide binding; lipid binding; receptor binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; immune system process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; lipid transport; positive regulation of tumor necrosis factor production; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; transport; defense response to bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; innate immune response; positive regulation of phagocytosis, engulfment; positive regulation of macrophage activation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage