catalog number :
MBS947443
products type :
ELISA Kit
products full name :
Mouse Protein S100-A9, S100A9 ELISA Kit
products short name :
[S100 calcium binding protein A9]
products name syn :
[Mouse Protein S100-A9 (S100A9) ELISA kit; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B) ; calgranulin B; S100 calcium binding protein A9]
other names :
[protein S100-A9; Protein S100-A9; protein S100-A9; S100 calcium-binding protein A9; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium binding protein A9 (calgranulin B); Calgranulin-B; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9]
products gene name :
[S100A9]
other gene names :
[S100a9; S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964; Cagb; Mrp14; MRP-14; p14]
uniprot entry name :
S10A9_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse S100A9. No significant cross-reactivity or interference between mouse S100A9 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.45 ng/ml -30 ng/ml. Sensitivity: The minimum detectable dose of mouse S100A9 is typically less than 0.11 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001268781.1
ncbi gb acc num :
NM_001281852.1
ncbi mol weight :
13,049 Da
uniprot summary :
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner. Protein type: Calcium-binding. Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; extracellular region; plasma membrane; nucleus. Molecular Function: arachidonic acid binding; antioxidant activity; RAGE receptor binding; zinc ion binding; microtubule binding; metal ion binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; immune system process; apoptosis; leukocyte chemotaxis; positive regulation of peptide secretion; chemotaxis; leukocyte migration during inflammatory response; regulation of inflammatory response; actin cytoskeleton reorganization; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; positive regulation of inflammatory response
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells