ELISA/assay

Human Syndecan-2, SDC2 ELISA Kit

MBS947364

96 Strip Wells

760 USD

ELISA Kit

Human Syndecan-2, SDC2 ELISA Kit

syndecan 2

Human Syndecan-2 (SDC2) ELISA kit; HSPG; HSPG1; SYND2; cell surface-associated heparan sulfate proteoglycan 1; fibroglycan; heparan sulfate proteoglycan 1; cell surface-associated; heparan sulfate proteoglycan core protein; syndecan prot; syndecan 2

syndecan-2; Syndecan-2; syndecan-2; fibroglycan; syndecan proteoglycan 2; heparan sulfate proteoglycan core protein; cell surface-associated heparan sulfate proteoglycan 1; heparan sulfate proteoglycan 1, cell surface-associated; syndecan 2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD_antigen: CD362

SDC2

SDC2; SDC2; HSPG; CD362; HSPG1; SYND2; HSPG1; SYND2; HSPG

SDC2_HUMAN

Human

201

This assay has high sensitivity and excellent specificity for detection of human SDC2. No significant cross-reactivity or interference between human SDC2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human syndecan-2 (SDC2) concentrations in serum, plasma, tissue homogenates. Detection Range: 93.75 pg/ml -6000 pg/ml.

Sensitivity: The minimum detectable dose of human SDC2 is typically less than 23.43 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

40548378

NP_002989.2

NM_002998.3

P34741

22,160 Da

Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!FGF Signaling Pathway 137989!!Malaria Pathway 152665!!Malaria Pathway 152657!!Proteogylcan Syndecan-mediated Signaling Events Pathway 137947!!Syndecan-2-mediated Signaling Events Pathway 138031!!TGF-beta Receptor Signaling Pathway 198774

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-2 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-2 expression has been detected in several different tumor types. [provided by RefSeq, Jul 2008]

Function: Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis

96 Strip Wells

760 USD