ELISA/assay

Human A disintegrin and metalloproteinase with thrombospondin motifs 2, ADAMTS2 ELISA Kit

MBS947192

48-Strip-Wells

510 USD

ELISA Kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 2, ADAMTS2 ELISA Kit

ADAM metallopeptidase with thrombospondin type 1 motif, 2

Human A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS2) ELISA kit; ADAM-TS2; ADAMTS-3; NPI; PCINP; PCPNI; hPCPNI; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 2; procollagen I N-proteinase; procollagen N-endopeptidase; ADAM metallopeptidase with thrombospondin type 1 motif; 2

A disintegrin and metalloproteinase with thrombospondin motifs 2 isoform 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 2; A disintegrin and metalloproteinase with thrombospondin motifs 2; procollagen I N-proteinase; procollagen N-endopeptidase; procollagen I/II amino propeptide-processing enzyme; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2; ADAM metallopeptidase with thrombospondin type 1 motif, 2; Procollagen I N-proteinase; PC I-NP; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase; pNPI

ADAMTS2

ADAMTS2; ADAMTS2; NPI; PNPI; PCINP; PCPNI; PCI-NP; PC I-NP; ADAM-TS2; ADAMTS-2; ADAMTS-3; PCINP; PCPNI; ADAM-TS 2; ADAM-TS2; ADAMTS-2; PC I-NP; pNPI

ATS2_HUMAN

Human

1211

This assay has high sensitivity and excellent specificity for detection of human ADAMTS2. No significant cross-reactivity or interference between human ADAMTS2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human ADAMTS2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

110825974

NP_055059.2

NM_014244.4

O95450

134,755 Da

Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262)

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene excises the N-propeptide of type I, type II and type V procollagens. Mutations in this gene cause Ehlers-Danlos syndrome type VIIC, a recessively inherited connective-tissue disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

ADAMTS2: Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis. Defects in ADAMTS2 are the cause of Ehlers-Danlos syndrome type 7C (EDS7C). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7C is marked by extremely fragile tissues, hyperextensible skin and easy bruising. Facial skin contains numerous folds, as in the cutis laxa syndrome. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.24.14; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Protease. Chromosomal Location of Human Ortholog: 5qter. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: skin development; collagen catabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; spermatogenesis; protein processing; proteolysis; lung development. Disease: Ehlers-danlos Syndrome, Type Vii, Autosomal Recessive

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800