catalog number :
MBS947027
products type :
ELISA Kit
products full name :
Rat C-X-C motif chemokine 9, CXCL9 ELISA Kit
products short name :
chemokine (C-X-C motif) ligand 9
products name syn :
Rat C-X-C motif chemokine 9 (CXCL9) ELISA kit; CMK; Humig; MIG; SCYB9; crg-10; monokine induced by gamma interferon; small inducible cytokine B9; chemokine (C-X-C motif) ligand 9
other names :
C-X-C motif chemokine 9; C-X-C motif chemokine 9; C-X-C motif chemokine 9; small-inducible cytokine B9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma; chemokine (C-X-C motif) ligand 9; Gamma-interferon-induced monokine; Monokine induced by interferon-gamma; HuMIG; MIG; Small-inducible cytokine B9
products gene name :
CXCL9
other gene names :
CXCL9; CXCL9; CMK; MIG; Humig; SCYB9; crg-10; CMK; MIG; SCYB9; HuMIG; MIG
uniprot entry name :
CXCL9_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of rat CXCL9. No significant cross-reactivity or interference between rat CXCL9 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: Typically less than 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002407.1
ncbi gb acc num :
NM_002416.1
ncbi mol weight :
14,019 Da
ncbi pathways :
CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)
ncbi summary :
The function of this gene has not been specifically defined; however, it is thought to be involved in T cell trafficking. This gene has been localized to 4q21 with INP10, which is also a member of the chemokine family of cytokines. [provided by RefSeq, Jul 2008]
uniprot summary :
CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; defense response; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; inflammatory response; chemotaxis; signal transduction; defense response to virus; positive regulation of myoblast differentiation; regulation of cell proliferation
size4 :
10x96-Strip-Wells