catalog number :
MBS946943
products type :
ELISA Kit
products full name :
Human ADP-ribosylation factor-like protein 8B, ARL8B ELISA Kit
products short name :
ADP-ribosylation factor-like 8B
products name syn :
Human ADP-ribosylation factor-like protein 8B (ARL8B) ELISA kit; ARL10C; FLJ10702; Gie1; ADP-ribosylation factor-like 10C; ADP-ribosylation factor-like 8B
other names :
ADP-ribosylation factor-like protein 8B; ADP-ribosylation factor-like protein 8B; ADP-ribosylation factor-like protein 8B; ADP-ribosylation factor-like 10C; ADP-ribosylation factor-like protein 10C; novel small G protein indispensable for equal chromosome segregation 1; ADP-ribosylation factor-like 8B; ADP-ribosylation factor-like protein 10C; Novel small G protein indispensable for equal chromosome segregation 1
products gene name :
ARL8B
other gene names :
ARL8B; ARL8B; Gie1; ARL10C; ARL10C; GIE1
uniprot entry name :
ARL8B_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ARL8B. No significant cross-reactivity or interference between Human ARL8B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Cycle. Protein Biological Process 3: Cell cycle
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARL8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARL8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARL8B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARL8B bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_060654.1
ncbi gb acc num :
NM_018184.2
ncbi mol weight :
21,539 Da
uniprot summary :
ARL8B: May play a role in lysosome motility (PubMed:16537643). May play a role in chromosome segregation (PubMed:15331635). Belongs to the small GTPase superfamily. Arf family. Protein type: G protein, monomeric, ARF; G protein, monomeric. Chromosomal Location of Human Ortholog: 3p26.1. Cellular Component: membrane; late endosome membrane; lysosomal membrane; cytoplasm; midbody; spindle midzone. Molecular Function: GTPase activity; GDP binding; GTP binding; beta-tubulin binding; alpha-tubulin binding. Biological Process: metabolic process; cell division; small GTPase mediated signal transduction; cell cycle; chromosome segregation
size4 :
10x96-Strip-Wells