This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Fibroblast growth factor 21, FGF21 ELISA Kit
catalog :
MBS946933
quantity :
24-Strip-Wells (LIMIT 1)
price :
240 USD
product information
catalog number :
MBS946933
products type :
ELISA Kit
products full name :
Mouse Fibroblast growth factor 21, FGF21 ELISA Kit
products short name :
[fibroblast growth factor 21]
products name syn :
[Mouse Fibroblast growth factor 21 (FGF21) ELISA kit; fibroblast growth factor 21]
other names :
[fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; FGF-21; fibroblast growth factor 21]
products gene name :
[FGF21]
other gene names :
[Fgf21; Fgf21; FGF-21]
uniprot entry name :
FGF21_MOUSE
reactivity :
Mouse
sequence length :
210
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse FGF21. No significant cross-reactivity or interference between mouse FGF21 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse FGF21 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
9910218
ncbi acc num :
NP_064397.1
ncbi gb acc num :
NM_020013.4
uniprot acc num :
Q9JJN1
ncbi mol weight :
23,237 Da
ncbi pathways :
ESC Pluripotency Pathways (198374); MAPK Signaling Pathway (83245); MAPK Signaling Pathway (456); Melanoma Pathway (83307); Melanoma Pathway (526); Pathways In Cancer (83298); Regulation Of Actin Cytoskeleton Pathway (198389); Regulation Of Actin Cytoskeleton Pathway (83286); Regulation Of Actin Cytoskeleton Pathway (500)
uniprot summary :
FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: growth factor activity; receptor binding; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; fibroblast growth factor receptor signaling pathway; positive regulation of cell proliferation
size1 :
24-Strip-Wells (LIMIT 1)
price1 :
240 USD
size2 :
48-Strip-Wells
price2 :
495
size3 :
96-Strip-Wells
price3 :
710
size4 :
5x96-Strip-Wells
price4 :
2705
size5 :
10x96-Strip-Wells
price5 :
5145
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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