catalog number :
MBS946642
products type :
ELISA Kit
products full name :
Human Protein DEK, DEK ELISA Kit
products short name :
[DEK oncogene]
products name syn :
[Human Protein DEK (DEK) ELISA kit; D6S231E; DEK oncogene (DNA binding) ; DEK oncogene]
other names :
[protein DEK isoform 2; Protein DEK; protein DEK; DEK oncogene (DNA binding); DEK proto-oncogene]
products gene name :
[DEK]
other gene names :
[DEK; DEK; D6S231E]
uniprot entry name :
DEK_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse DEFA6. No significant cross-reactivity or interference between mouse DEFA6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001128181.1
ncbi gb acc num :
NM_001134709.1
ncbi mol weight :
42,674 Da
ncbi summary :
This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
uniprot summary :
DEK: a ubiquitous protein with a SAP domain, which is found in a variety of nuclear proteins involved in transcription, DNA repair, RNA processing or apoptotic chromatin degradation. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA and is also involved in splice site selection during mRNA processing. A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemias (AML). Protein type: RNA splicing; Oncoprotein; Histone-binding; DNA replication; DNA-binding. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: contractile fiber; nucleus. Molecular Function: DNA binding; histone binding. Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; chromatin modification; viral genome replication; signal transduction
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells