product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Interferon alpha-inducible protein 27, mitochondrial, IFI27 ELISA Kit
catalog :
MBS946608
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS946608
products type :
ELISA Kit
products full name :
Human Interferon alpha-inducible protein 27, mitochondrial, IFI27 ELISA Kit
products short name :
interferon, alpha-inducible protein 27
products name syn :
Human Interferon alpha-inducible protein 27; mitochondrial (IFI27) ELISA kit; FAM14D; ISG12; ISG12A; P27; 2310061N23Rik; interferon; alpha-inducible protein 27
other names :
interferon alpha-inducible protein 27, mitochondrial isoform 1; Interferon alpha-inducible protein 27, mitochondrial; interferon alpha-inducible protein 27, mitochondrial; ISG12(a); 2310061N23Rik; interferon-stimulated gene 12a protein; interferon alpha-induced 11.5 kDa protein; interferon, alpha-inducible protein 27; Interferon alpha-induced 11.5 kDa protein; Interferon-stimulated gene 12a protein; ISG12(a)
products gene name :
IFI27
other gene names :
IFI27; IFI27; P27; ISG12; FAM14D; ISG12A; p27; ISG12(a)
uniprot entry name :
IFI27_HUMAN
reactivity :
Human
sequence length :
122
specificity :
This assay has high sensitivity and excellent specificity for detection of human IFI27. No significant cross-reactivity or interference between human IFI27 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: The minimum detectable dose of human IFI27 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFI27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFI27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFI27 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFI27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
194272171
ncbi acc num :
NP_001123552.1
ncbi gb acc num :
NM_001130080.2
uniprot acc num :
P40305
ncbi mol weight :
11,268 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104)
uniprot summary :
IFI27: Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9. Belongs to the IFI6/IFI27 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q32. Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane; nuclear inner membrane. Molecular Function: lamin binding. Biological Process: caspase activation; cytokine and chemokine mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; regulation of protein export from nucleus
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

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