Human Laminin subunit alpha-1, LAMA1 ELISA Kit | MyBioSource MBS946597 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Laminin subunit alpha-1, LAMA1 ELISA Kit

catalog :

MBS946597

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS946597

products type :

ELISA Kit

products full name :

Human Laminin subunit alpha-1, LAMA1 ELISA Kit

products short name :

laminin, alpha 1

products name syn :

Human Laminin subunit alpha-1 (LAMA1) ELISA kit; LAMA; ; laminin; alpha 1

other names :

laminin subunit alpha-1; Laminin subunit alpha-1; laminin subunit alpha-1; S-LAM alpha; laminin A chain; S-laminin subunit alpha; laminin-3 subunit alpha; laminin, alpha 1; Laminin A chain; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-laminin subunit alpha; S-LAM alpha

products gene name :

LAMA1

other gene names :

LAMA1; LAMA1; LAMA; S-LAM-alpha; LAMA; S-LAM alpha

uniprot entry name :

LAMA1_HUMAN

reactivity :

Human

sequence length :

3075

specificity :

This assay has high sensitivity and excellent specificity for detection of human LAMA1. No significant cross-reactivity or interference between human LAMA1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human LAMA1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

38788416

ncbi acc num :

NP_005550.2

ncbi gb acc num :

NM_005559.3

uniprot acc num :

P25391

ncbi mol weight :

337,084 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

ncbi summary :

This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]

uniprot summary :

LAMA1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix; Secreted. Chromosomal Location of Human Ortholog: 18p11.3. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; laminin-3 complex; laminin-1 complex; extracellular region; basement membrane; intercellular junction. Molecular Function: protein binding; extracellular matrix structural constituent; glycosphingolipid binding; receptor binding. Biological Process: regulation of cell adhesion; axon guidance; extracellular matrix organization and biogenesis; cell surface receptor linked signal transduction; regulation of embryonic development; morphogenesis of an epithelial sheet; establishment of epithelial cell polarity; cell adhesion; regulation of cell migration. Disease: Poretti-boltshauser Syndrome

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage