ELISA/assay

Human NADH-cytochrome b5 reductase 3, CYB5R3 ELISA Kit

MBS946553

48-Strip-Wells

510 USD

ELISA Kit

Human NADH-cytochrome b5 reductase 3, CYB5R3 ELISA Kit

cytochrome b5 reductase 3

Human NADH-cytochrome b5 reductase 3 (CYB5R3) ELISA kit; B5R; DIA1; NADH-cytochrome b5 reductase; OTTHUMP00000198574; OTTHUMP00000198665; diaphorase (NADH) (cytochrome b-5 reductase) ; cytochrome b5 reductase 3

NADH-cytochrome b5 reductase 3 isoform 1; NADH-cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3; diaphorase-1; NADH-cytochrome b5 reductase 3 soluble form; NADH-cytochrome b5 reductase 3 membrane-bound form; cytochrome b5 reductase 3; Diaphorase-1Cleaved into the following 2 chains:NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form

CYB5R3

CYB5R3; CYB5R3; B5R; DIA1; DIA1; B5R; Cytochrome b5 reductase

NB5R3_HUMAN

Human

301

This assay has high sensitivity and excellent specificity for detection of Human CYB5R3. No significant cross-reactivity or interference between Human CYB5R3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: 6.25 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Cholesterol biosynthesis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYB5R3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYB5R3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503327

NP_000389.1

NM_000398.6

P00387

34,235 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Metabolism Pathway (477135); Metabolism Of Vitamins And Cofactors Pathway (106249); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (106250); Vitamin C (ascorbate) Metabolism Pathway (106251); Gamma-linolenate Biosynthesis II (animals) Pathway (545309); Gamma-linolenate Biosynthesis II (animals) Pathway (138572)

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]

CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 1.6.2.2; Oxidoreductase. Chromosomal Location of Human Ortholog: 22q13.2. Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; hemoglobin complex; endoplasmic reticulum; mitochondrial inner membrane; cytoplasm; lipid particle. Molecular Function: FAD binding; cytochrome-b5 reductase activity; ADP binding; NAD binding; AMP binding. Biological Process: vitamin metabolic process; blood circulation; L-ascorbic acid metabolic process; cholesterol biosynthetic process; water-soluble vitamin metabolic process. Disease: Methemoglobinemia Due To Deficiency Of Methemoglobin Reductase

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800