catalog number :
MBS946537
products type :
ELISA Kit
products full name :
Bovine Pulmonary surfactant-associated protein D, SFTPD ELISA Kit
products short name :
surfactant protein D
products name syn :
Bovine Pulmonary surfactant-associated protein D (SFTPD) ELISA kit; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary; surfactant protein D
other names :
pulmonary surfactant-associated protein D; Pulmonary surfactant-associated protein D; pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D; surfactant, pulmonary-associated protein D; surfactant protein D; Lung surfactant protein D
products gene name :
SFTPD
other gene names :
SFTPD; SFTPD; SP-D; COLEC7; SFTP4; PSP-D; SP-D
uniprot entry name :
SFTPD_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine SFTPD. No significant cross-reactivity or interference between bovine SFTPD and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.88 ng/ml -120 ng/ml. Sensitivity: 0.47 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFTPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFTPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPD bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_851369.1
ncbi gb acc num :
NM_181026.2
ncbi mol weight :
37,405 Da
ncbi pathways :
Phagosome Pathway (153904); Phagosome Pathway (153859)
uniprot summary :
Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
size4 :
10x96-Strip-Wells