catalog number :
MBS946511
products type :
ELISA Kit
products full name :
Mouse oxidized high density lipoprotein, Ox-HDL ELISA Kit
products short name :
oxidized high density lipoprotein (Ox-HDL)
products name syn :
Mouse oxidized high density lipoprotein (Ox-HDL) ELISA Kit; oxidized high density lipoprotein (Ox-HDL)
products gene name :
Ox-HDL
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse Ox-HDL. No significant cross-reactivity or interference between mouse Ox-HDL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse Ox-HDL is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse Ox-HDL concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ox-HDL. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Ox-HDL. The competitive inhibition reaction is launched between with pre-coated Ox-HDL and Ox-HDL in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Ox-HDL in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells