catalog number :
MBS946336
products type :
ELISA Kit
products full name :
Mouse CAMPATH-1 antigen, CD52 ELISA Kit
products short name :
CD52 molecule
products name syn :
Mouse CAMPATH-1 antigen (CD52) ELISA kit; CDW52; CD52 antigen; CD52 antigen (CAMPATH-1 antigen) ; CDW52 antigen (CAMPATH-1 antigen) ; CD52 molecule
other names :
CAMPATH-1 antigen; CAMPATH-1 antigen; CAMPATH-1 antigen; lymphocyte differentiation antigen B7; CD52 antigen; Lymphocyte differentiation antigen B7; CD_antigen: CD52
products gene name :
CD52
other gene names :
Cd52; Cd52; B7; MB7; CLS1; B7-Ag; AI463198; CAMPATH-1; Cdw52; Mb7
uniprot entry name :
CD52_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse CD52. No significant cross-reactivity or interference between mouse CD52 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of mouse CD52 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD52 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD52 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_038734.1
ncbi gb acc num :
NM_013706.2
ncbi mol weight :
7,798 Da
uniprot summary :
CD52: May play a role in carrying and orienting carbohydrate, as well as having a more specific role. Protein type: Membrane protein, GPI anchor; Membrane protein, integral. Cellular Component: membrane; plasma membrane
size4 :
10x96-Strip-Wells