catalog number :
MBS946326
products type :
ELISA Kit
products full name :
Rat Amyloid beta A4 protein, APP ELISA Kit
products short name :
[amyloid beta (A4) precursor protein]
products name syn :
[Rat Amyloid beta A4 protein (APP) ELISA kit; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN2; OTTHUMP00000096096; amyloid beta A4 protein; beta-amyloid peptide; cerebral vascular amyloid peptide; peptidase nexin-II; protease nexin-II; amyloid beta (A4) precursor protein]
other names :
[Amyloid beta A4 protein; Amyloid beta A4 protein; ABPP; APP; Alzheimer disease amyloid A4 protein homologCleaved into the following 6 chains:Soluble APP-beta; S-APP-beta; CTF-alpha; Beta-amyloid protein 42Alternative name(s):Beta-APP42]
products gene name :
[APP]
other gene names :
[APP; APP; S-APP-beta]
uniprot entry name :
A4_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat APP. No significant cross-reactivity or interference between rat APP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml-1000 ng/ml. Sensitivity: Typically less than 3.9 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
6,300 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O)
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells